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Prolactin inhibits oocyte release after gonadotropin stimulation in the rat: Putative mechanism involving ovarian production of beta-endorphin and prostaglandin 

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Presentation on theme: "Prolactin inhibits oocyte release after gonadotropin stimulation in the rat: Putative mechanism involving ovarian production of beta-endorphin and prostaglandin "— Presentation transcript:

1 Prolactin inhibits oocyte release after gonadotropin stimulation in the rat: Putative mechanism involving ovarian production of beta-endorphin and prostaglandin  Fernanda Polisseni, M.D., Alicia G. Faletti, Ph.D., Virginia M. Pereira, M.Sc., Adelina M. Reis, Ph.D., Aroldo F. Camargos, M.D., Ph.D., Fernando M. Reis, M.D., Ph.D.  Fertility and Sterility  Volume 83, Issue 4, Pages (April 2005) DOI: /j.fertnstert Copyright © 2005 American Society for Reproductive Medicine Terms and Conditions

2 FIGURE 1 Effects of prolactin (PRL) (100 or 200 μg) and PRL + naloxone (200 μg each) on the number of oocytes recovered in the oviducts of superovulated rats. *P<.05; **P<.01 vs. control (Student-Newman-Keuls test). Other comparisons between treatments were not statistically significant. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2005 American Society for Reproductive Medicine Terms and Conditions

3 FIGURE 2 Representative photomicrographs of preovulatory follicles from rats treated with (a) saline vehicle or (b) 200 μg prolactin (PRL) 4 hours after hCG injection for ovulation induction. Magnification was ×200. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2005 American Society for Reproductive Medicine Terms and Conditions

4 FIGURE 3 Effects of prolactin (PRL) (100 or 200 ng/mL) on the isolated rat ovary in vitro. (A), Beta-endorphin release; (B), prostaglandin E2 release; (C), NO synthase activity (expressed as picomoles of NO per gram w.w. per minute); and (D), NO2−/NO3− release (expressed by the NO2− concentration after NO3− reduction). *P<.05 vs. control (Student-Newman-Keuls test). Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2005 American Society for Reproductive Medicine Terms and Conditions


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