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Teresa Asensio, MD, Jesus F

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1 Novel plant pathogenesis-related protein family involved in food allergy 
Teresa Asensio, MD, Jesus F. Crespo, PhD, MD, Rosa Sanchez-Monge, PhD, Gema Lopez-Torrejon, PhD, Maria L. Somoza, MD, Julia Rodriguez, MD, Gabriel Salcedo, PhD  Journal of Allergy and Clinical Immunology  Volume 114, Issue 4, Pages (October 2004) DOI: /j.jaci Copyright © 2004 American Academy of Allergy, Asthma and Immunology Terms and Conditions

2 Fig 1 A, HPLC fractionation of the melon juice extract. Arrow points to the peak corresponding to Cuc m 3. B, Protein staining and IgE immunodetection with a serum pool from melon-allergic patients of melon pulp (lane 1) and juice (lane 2) extract and of purified Cuc m 3 (lane 3), separated by SDS-PAGE. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2004 American Academy of Allergy, Asthma and Immunology Terms and Conditions

3 Fig 2 A, HPLC fractionation of peptides produced by endoproteinase Lys-C hydrolysis of purified Cuc m 3. The N-terminal amino acid sequence of each of 3 major peptides is indicated. Alignment of the N-terminal amino acid of peptides derived from Cuc m 3 and the corresponding internal region of PR-1 proteins from grape (accession no. Q7XAJ6) and cucumber (accession no. Q8S3W2) (B) and of insect venom allergen Vesp c 5 (accession no. P35782) from Vespa cabro (European hornet) (C). Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2004 American Academy of Allergy, Asthma and Immunology Terms and Conditions

4 Fig 3 A, IgE binding to Cuc m 3 of 17 individual sera from patients with melon allergy. BSA (NG1) and a class II chitinase from chestnut seed (NG2) were used as negative controls. B, ELISA inhibition with melon juice and pulp extracts in the solid phase and purified Cuc m 3 as inhibitor. A serum pool from melon allergic patients was used in the assays. Means (n=3) and SDs (bars) are represented in both panels. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2004 American Academy of Allergy, Asthma and Immunology Terms and Conditions

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