Presentation is loading. Please wait.

Presentation is loading. Please wait.

M cell targeting with Aleuria aurantia lectin as a novel approach for oral allergen immunotherapy  Franziska Roth-Walter, PhD, Isabella Schöll, PhD, Eva.

Published byEmmanuelle Germain Modified over 5 years ago

Similar presentations

Presentation on theme: "M cell targeting with Aleuria aurantia lectin as a novel approach for oral allergen immunotherapy  Franziska Roth-Walter, PhD, Isabella Schöll, PhD, Eva."— Presentation transcript:

1 M cell targeting with Aleuria aurantia lectin as a novel approach for oral allergen immunotherapy 
Franziska Roth-Walter, PhD, Isabella Schöll, PhD, Eva Untersmayr, MD, Renate Fuchs, PhD, George Boltz-Nitulescu, PhD, Andrea Weissenböck, PhD, Otto Scheiner, PhD, Franz Gabor, PhD, Erika Jensen-Jarolim, MD  Journal of Allergy and Clinical Immunology  Volume 114, Issue 6, Pages (December 2004) DOI: /j.jaci Copyright © 2004 American Academy of Allergy, Asthma and Immunology Terms and Conditions

2 Fig 1 A, Adhesion of bare microspheres (1), AAL microspheres (2), or WGA microspheres (3) to Caco2 intestinal epithelial cells. Caco2 cells were incubated with the different types of fluorescein-cadaverine–labeled microspheres. Plasma membranes were localized with rabbit antialkaline phosphatase antibody and Alexa568 conjugated antirabbit IgG. Nuclei were stained with Hoechst dye. B, AAL microparticles (black bars) and WGA (gray bars) bound substantially better to a Caco2 monolayer than bare microspheres (white bars). The intestinal epithelial cell line Caco2 was grown in monolayers and incubated at 4°C with different microsphere preparations containing FITC-cadaverin. Cells with bound microspheres were dissolved and mean fluorescence (n=3) detected at 485/535 nm. rFU, Relative fluorescence units. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2004 American Academy of Allergy, Asthma and Immunology Terms and Conditions

3 Fig 2 Birch pollen–sensitized mice (n=8 per group) were treated orally with BP-loaded microparticles functionalized with AAL (black columns) or WGA (gray columns) or without functionalization (white columns). Sera before and after treatment were analyzed for BP-specific IgG1 titer, IgE, and IgA titers (μg/mL serum) in ELISA. ∗P < .05. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2004 American Academy of Allergy, Asthma and Immunology Terms and Conditions

4 Fig 3 Mice (n=8 per group) were sensitized to BP and afterward treated orally with BP-loaded microparticles. Splenocytes proliferated in all groups specifically to BP, but not with the control allergens of grass pollen (GP). Feedings of these mice were performed with BP-loaded AAL microparticles (dark gray columns), WGA microparticles (gray columns), or uncoated microparticles (white columns). cpm, Counts per minute. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2004 American Academy of Allergy, Asthma and Immunology Terms and Conditions

5 Fig 4 Supernatants of splenocytes of BP-sensitized mice (n=8 per group), which were treated orally with BP-loaded microparticles, were analyzed for cytokine contents. WGA-coated and AAL-coated microparticles produced significantly more IL-10 and IL-4 than feedings with bare, BP-loaded microparticles (MSBP). For IFN-γ, only AAL microspheres (AALMS) produced significant elevation of this cytokine. WGAMS, WGA microspheres. ∗P < .05. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2004 American Academy of Allergy, Asthma and Immunology Terms and Conditions

Download ppt "M cell targeting with Aleuria aurantia lectin as a novel approach for oral allergen immunotherapy  Franziska Roth-Walter, PhD, Isabella Schöll, PhD, Eva."

Similar presentations

Secretory IgA induces antigen-independent eosinophil survival and cytokine production without inducing effector functions  Kathleen R. Bartemes, BA, Kate.

Secretory IgA induces antigen-independent eosinophil survival and cytokine production without inducing effector functions  Kathleen R. Bartemes, BA, Kate.
Detection of novel occupational wood allergens in locust wood dust (Robinia pseudoacacia L.)  Sabine Kespohl, PhD, Rolf Merget, MD, Axel Overlack, MD,

Detection of novel occupational wood allergens in locust wood dust (Robinia pseudoacacia L.)  Sabine Kespohl, PhD, Rolf Merget, MD, Axel Overlack, MD,
Kiyoshi Yamazaki, MD, Joseph A. Murray, MD, Hirohito Kita, MD 

Kiyoshi Yamazaki, MD, Joseph A. Murray, MD, Hirohito Kita, MD 
Repeated low-dose intradermal allergen injection suppresses allergen-induced cutaneous late responses  Giuseppina Rotiroti, MD, Mohamed Shamji, PhD, Stephen.

Repeated low-dose intradermal allergen injection suppresses allergen-induced cutaneous late responses  Giuseppina Rotiroti, MD, Mohamed Shamji, PhD, Stephen.
Birch pollen–related food allergy: Clinical aspects and the role of allergen-specific IgE and IgG4 antibodies  Marija Geroldinger-Simic, MD, Thomas Zelniker,

Birch pollen–related food allergy: Clinical aspects and the role of allergen-specific IgE and IgG4 antibodies  Marija Geroldinger-Simic, MD, Thomas Zelniker,
Cor a 1–reactive T cells and IgE are predominantly cross-reactive to Bet v 1 in patients with birch pollen–associated food allergy to hazelnut  Claudia.

Cor a 1–reactive T cells and IgE are predominantly cross-reactive to Bet v 1 in patients with birch pollen–associated food allergy to hazelnut  Claudia.
Relevance of specific IgE antibody titer to the prevalence, severity, and persistence of asthma among 19-year-olds in northern Sweden  Matthew S. Perzanowski,

Relevance of specific IgE antibody titer to the prevalence, severity, and persistence of asthma among 19-year-olds in northern Sweden  Matthew S. Perzanowski,
Mapping of conformational IgE epitopes on Phl p 5a by using mimotopes from a phage display library  Brigitte Hantusch, MSc, Sigurd Krieger, TA, Eva Untersmayr,

Mapping of conformational IgE epitopes on Phl p 5a by using mimotopes from a phage display library  Brigitte Hantusch, MSc, Sigurd Krieger, TA, Eva Untersmayr,
High-titer IgE antibody specific for pollen allergens in northern California is associated with both wheezing and total serum IgE  Elizabeth A. Erwin,

High-titer IgE antibody specific for pollen allergens in northern California is associated with both wheezing and total serum IgE  Elizabeth A. Erwin,
Antacid medication inhibits digestion of dietary proteins and causes food allergy  Eva Untersmayr, MD, Isabella Schöll, MSc, Ines Swoboda, PhD, Waltraud.

Antacid medication inhibits digestion of dietary proteins and causes food allergy  Eva Untersmayr, MD, Isabella Schöll, MSc, Ines Swoboda, PhD, Waltraud.
IgE-mediated systemic anaphylaxis and impaired tolerance to food antigens in mice with enhanced IL-4 receptor signaling  Clinton B. Mathias, PhD, Suejy.

IgE-mediated systemic anaphylaxis and impaired tolerance to food antigens in mice with enhanced IL-4 receptor signaling  Clinton B. Mathias, PhD, Suejy.
Advances in food allergy in 2015

Advances in food allergy in 2015
Designing hypoallergenic derivatives for allergy treatment by means of in silico mutation and screening  Theresa Thalhamer, PhD, Heidi Dobias, MSc, Tatjana.

Designing hypoallergenic derivatives for allergy treatment by means of in silico mutation and screening  Theresa Thalhamer, PhD, Heidi Dobias, MSc, Tatjana.
Santa Jeremy Ono, BA, PhD, Mark B. Abelson, MD 

Santa Jeremy Ono, BA, PhD, Mark B. Abelson, MD 
Maternal allergy increases susceptibility to offspring allergy in association with TH2- biased epigenetic alterations in a mouse model of peanut allergy 

Maternal allergy increases susceptibility to offspring allergy in association with TH2- biased epigenetic alterations in a mouse model of peanut allergy 
Giant ragweed specific immunotherapy is not effective in a proportion of patients sensitized to short ragweed: Analysis of the allergenic differences.

Giant ragweed specific immunotherapy is not effective in a proportion of patients sensitized to short ragweed: Analysis of the allergenic differences.
Differential cytokine induction by the human skin–associated autoallergen thioredoxin in sensitized patients with atopic dermatitis and healthy control.

Differential cytokine induction by the human skin–associated autoallergen thioredoxin in sensitized patients with atopic dermatitis and healthy control.
Relevance of specific IgE antibody titer to the prevalence, severity, and persistence of asthma among 19-year-olds in northern Sweden  Matthew S. Perzanowski,

Relevance of specific IgE antibody titer to the prevalence, severity, and persistence of asthma among 19-year-olds in northern Sweden  Matthew S. Perzanowski,
Cor a 1–reactive T cells and IgE are predominantly cross-reactive to Bet v 1 in patients with birch pollen–associated food allergy to hazelnut  Claudia.

Cor a 1–reactive T cells and IgE are predominantly cross-reactive to Bet v 1 in patients with birch pollen–associated food allergy to hazelnut  Claudia.
Development and characterization of a recombinant, hypoallergenic, peptide-based vaccine for grass pollen allergy  Margarete Focke-Tejkl, PhD, Milena.

Development and characterization of a recombinant, hypoallergenic, peptide-based vaccine for grass pollen allergy  Margarete Focke-Tejkl, PhD, Milena.

Similar presentations

Ads by Google