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IHC detection of p-mTOR Ser 2448 in subcortical white matter during postnatal rat CNS development. IHC detection of p-mTOR Ser 2448 in subcortical white.

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Presentation on theme: "IHC detection of p-mTOR Ser 2448 in subcortical white matter during postnatal rat CNS development. IHC detection of p-mTOR Ser 2448 in subcortical white."— Presentation transcript:

1 IHC detection of p-mTOR Ser 2448 in subcortical white matter during postnatal rat CNS development.
IHC detection of p-mTOR Ser 2448 in subcortical white matter during postnatal rat CNS development. A–C , Fixed, paraffin-embedded sections from postnatal day 14 ( A ), day 18 ( B ), or day 21 ( C ) were used for IHC staining of p-mTOR Ser 2448 (detected with vector-red). D , Image (40×) of p-mTOR staining in the WM at P21. E , Control d 21 section stained with secondary antibody alone. Sections were counter-stained with hematoxylin. F , 40× image of p-mTOR staining in the WM at d 21. Immunostaining of p-mTOR (TRITC), MBP (FITC), and DAPI in corpus callosum. Panels show two fields with colocalization of p-mTOR and MBP (arrowheads). G , Immunostaining of p-mTOR (TRITC), GFAP (FITC), and DAPI in corpus callosum. Panels show GFAP and p-mTOR in separate cells in two fields. William A. Tyler et al. J. Neurosci. 2009;29: ©2009 by Society for Neuroscience

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