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Genotype analysis of the neuropeptide Y (NPY) Y1 and NPY Y5 receptor genes in gonadotropin-releasing hormone–dependent precocious gonadarche Mandi Barker-Gibb, Ph.D., Tony M Plant, Ph.D., Carlie White, B.Sc., Peter A Lee, M.D., Ph.D., Selma Feldman Witchel, M.D. Fertility and Sterility Volume 82, Issue 2, Pages (August 2004) DOI: /j.fertnstert
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Figure 1 (A) Illustration of NPY Y1 (blue) and Y5 (green) receptor genes. Coding regions of exons are indicated by filled boxes. Noncoding regions of exons are indicated by hatched boxes. The small arrow indicates the position of the G→A variation in the Y5 receptor gene. (B) Single-stranded conformation polymorphism analysis. The gene-specific primers amplified the coding region of exon 2 of the Y5 receptor gene. DNA samples from four different patients with precocious gonadarche are illustrated in lane 1 through lane 4. The migration of the normal (G, black) allele is shown in lanes 1, 2, and 4, and lane 3 shows the migration of the variant (A, green) allele. (C) Electropherogram showing sequencing of the unique single-stranded conformation polymorphism band. The arrow indicates the position of the G→A transition located in exon 2 of the Y5 receptor gene. Barker-Gibb. NPY receptors and precocious gonadarche. Fertil Steril 2004. Fertility and Sterility , DOI: ( /j.fertnstert )
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