Presentation is loading. Please wait.

Presentation is loading. Please wait.

Modulation of monocyte adherence to endothelial cells by endothelin-1 involvement of Src (p60src) and JAK1-like kinases  Lindsey J. Chisholm, MB, BS,

Published by혜선 선우 Modified over 5 years ago

Similar presentations

Presentation on theme: "Modulation of monocyte adherence to endothelial cells by endothelin-1 involvement of Src (p60src) and JAK1-like kinases  Lindsey J. Chisholm, MB, BS,"— Presentation transcript:

1 Modulation of monocyte adherence to endothelial cells by endothelin-1 involvement of Src (p60src) and JAK1-like kinases  Lindsey J. Chisholm, MB, BS, Peter S. Dovgan, MD, Devendra K. Agrawal, PhD, Patrick E. McGregor, MD, John D. Edwards, MD  Journal of Vascular Surgery  Volume 23, Issue 2, Pages (February 1996) DOI: /S (96) Copyright © 1996 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

2 Fig. 1 Effect of ET-1 (10-7 mol/L) on monocyte adherence to HUVECs incubated with ET-1 for various time periods. Percent increase above baseline monocyte adherence (in absence of ET-1) was calculated. Values are mean ± SEM, n = 5-8. *p Value <0.05. Journal of Vascular Surgery  , DOI: ( /S (96) ) Copyright © 1996 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

3 Fig. 2 Dose response effect of ET-1 on monocyte adherence to HUVECs. Monocytes were incubated with various doses of ET-1 for 4 hours under defined experimental conditions. Values are mean ± SEM, n = 7. Journal of Vascular Surgery  , DOI: ( /S (96) ) Copyright © 1996 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

4 Fig. 3 Effect of phosphoramidon (10-5 mol/L) on ET-1-induced monocyte adherence to HUVECs. HUVECs were preincubated with phosphoramidon for 15 minutes before incubation with ET mol/L or ET mol/L for 4 hours. Values are mean ± SEM, n = 4. Journal of Vascular Surgery  , DOI: ( /S (96) ) Copyright © 1996 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

5 Fig. 4 Effect of tyrosine kinase inhibitors on ET-1-induced monocyte adherence to HUVECs. HUVECs were incubated with genistein (G) 10-4 mol/L or herbimycin A (H) 8.75 × 10-7 mol/L for 15 minutes before addition of ET mol/L or ET mol/L, HUVECs were then incubated for 4 hours at 37º C in 95% air/5% CO2 in humidified chamber. Values are mean ± SEM, n = 6-8. p < 0.05; **p < 0.01. Journal of Vascular Surgery  , DOI: ( /S (96) ) Copyright © 1996 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

6 Fig. 5 Effect of ET-1 in presence and absence of endothelin-B receptor antagonist IRL 1038 (10-5 mol/L) on monocyte adherence to HUVECs. HUVECs were incubated with ETB receptor antagonist for 15 minutes before incubation with ET-1 at various doses. Values are mean ± SEM, n = 7. Journal of Vascular Surgery  , DOI: ( /S (96) ) Copyright © 1996 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

7 Fig. 6 Time course for effect of ET-1 (10-8 mol/L) on production of tyrosine phosphorylated proteins by HUVECs as detected by immunoblotting with PY20. Lanes marked G and H are samples where HUVECs were incubated with tyrosine kinase inhibitor genistein (G) 10-4 mol/L or herbimycin A (H) 8.75 × 10-7 mol/L for 10 minutes before addition of ET mol/L for 5 minutes. Data are representative of three experiments. Journal of Vascular Surgery  , DOI: ( /S (96) ) Copyright © 1996 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

8 Fig. 7 Dose response effect of ET-1 on production of tyrosine phosphorylated proteins by HUVECs as detected by immunoblotting with PY20. Lanes marked G and H are samples of HUVECs incubated with tyrosine kinase inhibitors genistein (G) 10-4 mol/L or herbimycin A (H) 8.75 × 10-7 mol/L for 10 minutes before addition of ET mol/L for 5 minutes. Data are representative of two individual experiments. Journal of Vascular Surgery  , DOI: ( /S (96) ) Copyright © 1996 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

9 Fig. 8 Effect of ETA and ETB receptor antagonists on tyrosine phosphorylated proteins as detected by immunoblotting with PY20. HUVECs were incubated with ETA receptor (5 × 10-8 mol/L) and ETB receptor antagonist (5 × 10-7 mol/L) for 10 minutes alone or before addition of ET mol/L for 5 minutes of incubation. Lane marked G shows effect of incubating HUVECs with genistein (G) 10-4 mol/L for 10 minutes before addition of ET mol/L for 5 minutse. Journal of Vascular Surgery  , DOI: ( /S (96) ) Copyright © 1996 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

10 Fig. 9 Immunoblotting of ET-1-(10-8 mol/L) stimulated HUVECs with antibody to src, JAK1, or JAK2 or appropriate control peptide (CP). This is representative of four separate experiments. Journal of Vascular Surgery  , DOI: ( /S (96) ) Copyright © 1996 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

11 Fig. 10 Immunoprecipitation of ET-1-stimulated and control HUVECs with antibody to p60src followed by immunoblotting with PY20 or antibody to c-src. This is representative of three separate experiments. Journal of Vascular Surgery  , DOI: ( /S (96) ) Copyright © 1996 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

12 Fig. 11 Immunoprecipitation of ET-1-stimulated and control HUVECs with antibody to JAK1 followed by immunoblotting with PY20 or antibody to JAK1. This is representative of three separate experiments. Journal of Vascular Surgery  , DOI: ( /S (96) ) Copyright © 1996 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Download ppt "Modulation of monocyte adherence to endothelial cells by endothelin-1 involvement of Src (p60src) and JAK1-like kinases  Lindsey J. Chisholm, MB, BS,"

Similar presentations

Endothelial cell seeding reduces thrombogenicity of Dacron grafts in humans Per Örtenwall, MD, PhD *, Hans Wadenvik, MD, PhD **, Jack Kutti, MD, PhD **,

Endothelial cell seeding reduces thrombogenicity of Dacron grafts in humans Per Örtenwall, MD, PhD *, Hans Wadenvik, MD, PhD **, Jack Kutti, MD, PhD **,
The role of arteriovenous shunts in the pathogenesis of varicose veins Howard C. Baron, M.D., Sebastiano Cassaro, M.D. Journal of Vascular Surgery Volume.

The role of arteriovenous shunts in the pathogenesis of varicose veins Howard C. Baron, M.D., Sebastiano Cassaro, M.D. Journal of Vascular Surgery Volume.
Platelet survival and serotonin content after placement of arterial prostheses in dogs: Effects of neointimal coverage and high- and low-dose aspirin G.Patrick.

Platelet survival and serotonin content after placement of arterial prostheses in dogs: Effects of neointimal coverage and high- and low-dose aspirin G.Patrick.
Silent myocardial ischemia monitoring predicts late as well as perioperative cardiac events in patients undergoing vascular surgery Peter F. Pasternack,

Silent myocardial ischemia monitoring predicts late as well as perioperative cardiac events in patients undergoing vascular surgery Peter F. Pasternack,
The effect of inguinal lymphatic manipulation on regional lymph flow patterns Jeffrey R. Rubin, MD, Lisa B. Eberlin, MD Journal of Vascular Surgery Volume.

The effect of inguinal lymphatic manipulation on regional lymph flow patterns Jeffrey R. Rubin, MD, Lisa B. Eberlin, MD Journal of Vascular Surgery Volume.
A review of 100 consecutive reconstructions of the distal vertebral artery for embolic and hemodynamic disease  Ramon Berguer, MD, PhD, Mark D. Morasch,

A review of 100 consecutive reconstructions of the distal vertebral artery for embolic and hemodynamic disease  Ramon Berguer, MD, PhD, Mark D. Morasch,
Aortic injury occurring after minor trauma in ankylosing spondylitis

Aortic injury occurring after minor trauma in ankylosing spondylitis
Heparin-coated catheters and heparin-induced thrombocytopenia

Heparin-coated catheters and heparin-induced thrombocytopenia
Do patients with the heparin-induced thrombocytopenia syndrome have heparin-specific antibodies?  John G. Adams, MD, Loren J. Humphrey, MD, PhD, Xinchao.

Do patients with the heparin-induced thrombocytopenia syndrome have heparin-specific antibodies?  John G. Adams, MD, Loren J. Humphrey, MD, PhD, Xinchao.
Heparin inhibits thrombin-induced mitogen-activated protein kinase signaling in arterial smooth muscle cells  Ulf Hedin, MD, PhD, Günter Daum, PhD, Alexander.

Heparin inhibits thrombin-induced mitogen-activated protein kinase signaling in arterial smooth muscle cells  Ulf Hedin, MD, PhD, Günter Daum, PhD, Alexander.
Sandhya K. Balaram, MD, Devendra K. Agrawal, PhD, R

Sandhya K. Balaram, MD, Devendra K. Agrawal, PhD, R
Mitogen-activated protein kinase activation: An alternate signaling pathway for sustained vascular smooth muscle contraction  Aaron M. Epstein, MD, Douglas.

Mitogen-activated protein kinase activation: An alternate signaling pathway for sustained vascular smooth muscle contraction  Aaron M. Epstein, MD, Douglas.
Domain-dependent action of urokinase on smooth muscle cell responses

Domain-dependent action of urokinase on smooth muscle cell responses
Granulocyte-macrophage colony-stimulating factor and IL-5 activate mitogen-activated protein kinase through Jak2 kinase and phosphatidylinositol 3-kinase.

Granulocyte-macrophage colony-stimulating factor and IL-5 activate mitogen-activated protein kinase through Jak2 kinase and phosphatidylinositol 3-kinase.
Mycotic axillary artery aneurysm

Mycotic axillary artery aneurysm
Sandhya K. Balaram, MD, Devendra K. Agrawal, PhD, R

Sandhya K. Balaram, MD, Devendra K. Agrawal, PhD, R
Marilyn J. Cipolla, MS, Christian T. Harker, PhD, John M. Porter, MD 

Marilyn J. Cipolla, MS, Christian T. Harker, PhD, John M. Porter, MD 
A new valvulotome for in situ bypass grafts

A new valvulotome for in situ bypass grafts
The incidence of heparin-induced antibodies in patients undergoing vascular surgery: A prospective study  Mark R. Jackson, MD, David L. Gillespie, MD,

The incidence of heparin-induced antibodies in patients undergoing vascular surgery: A prospective study  Mark R. Jackson, MD, David L. Gillespie, MD,
Β-Adrenergic regulation of the eosinophil respiratory burst as detected by lucigenin- dependent luminescence  Nicholas E. Hadjokas, PhD a,b, Joseph J.

Β-Adrenergic regulation of the eosinophil respiratory burst as detected by lucigenin- dependent luminescence  Nicholas E. Hadjokas, PhD a,b, Joseph J.

Similar presentations

Ads by Google