1. Human follicular fluid stimulates the sperm acrosome reaction by interacting with the γ- aminobutyric acid receptors 
Nunziatina Burrello, Ph.D., Enzo Vicari, M.D., Letizia D'Amico, Ph.D., Annalisa Satta, Ph.D., Rosario D'Agata, M.D., Aldo E. Calogero, M.D. 
Fertility and Sterility 
Volume 82, Pages (October 2004)
DOI: /j.fertnstert
Copyright © 2004 American Society for Reproductive Medicine Terms and Conditions

2. FIGURE 1
Effects of increasing concentrations of human follicular fluid on the acrosome reaction of human spermatozoa obtained from 10 normozoospermic subjects. *P<.05 vs. zero; †P<.05 vs. follicular fluid 20%, 30%, and 50%; ‡P<.05 vs. follicular fluid 10%, 30%, and 50%.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2004 American Society for Reproductive Medicine Terms and Conditions

3. FIGURE 2
Effects of bicuculline, a GABAA receptor antagonist, and saclofen, a GABAB receptor antagonist, on the acrosome reaction of human spermatozoa induced by a maximally stimulatory concentration of human follicular fluid (30%) obtained from eight normozoospermic subjects. *P<.05 vs. Basal (no treatment control); †P<.05 vs. follicular fluid 30%.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2004 American Society for Reproductive Medicine Terms and Conditions

4. FIGURE 3
Effects of picrotoxin, a chloride channel blocker, and saclofen, a GABAB receptor antagonist, on the acrosome reaction of human spermatozoa induced by a maximally stimulatory concentration of human follicular fluid (30%) obtained from seven normozoospermic subjects. Low = picrotoxin 10 μM + saclofen 1 μM; medium = picrotoxin 100 μM + saclofen 3 μM; high = picrotoxin 300 μM + saclofen 10 μM. *P<.05 vs. Basal (no treatment control); †P<.05 vs. follicular fluid 30%.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2004 American Society for Reproductive Medicine Terms and Conditions

5. FIGURE 4
Effects of picrotoxin, a chloride channel blocker, and verapamil, a calcium channel blocker, on the acrosome reaction of human spermatozoa induced by a maximally stimulatory concentration of human follicular fluid (30%) obtained from nine normozoospermic subjects. Low = picrotoxin 10 μM + verapamil 1 μM; medium = picrotoxin 100 μM + verapamil 3 μM; high = picrotoxin 300 μM + verapamil 10 μM. *P<.05 vs. Basal (no treatment control); †P<.05 vs. follicular fluid 30%.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2004 American Society for Reproductive Medicine Terms and Conditions