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Polyamine concentration (nmol/mg protein)

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Presentation on theme: "Polyamine concentration (nmol/mg protein)"— Presentation transcript:

1 Polyamine concentration (nmol/mg protein)
B Control SSAT SPDS SPMS siRNA Doxycycline Control si ODC kDa Doxorubicin kDa Doxorubicin (Hrs): 58 58 DNp73β DNp73 46 30 Egfp Actin odc ssat RT-PCR gapdh spds spms gapdh C D Polyamine concentration (nmol/mg protein) H1299 HCT116 kDa Doxorubicin (Hrs): 58 DNp73β 46 Actin paox gapdh Supplementary Figure 1 Bunjobpol et al.

2 Supplementary Figure 2 Bunjobpol et al.
C Vector FosB mutant paox RLU kDa CHX (Hrs) 58 DNp73β 46 FosB 30 46 Actin % DNp73 remaining Time after cycloheximide (Hrs) B D Vector c-Jun CHX (Hrs) Actin DNp73β siRNA % DNp73 remaining Time after cycloheximide (Hrs) JunB Actin DNp73β Vector CHX (Hrs) Time after cycloheximide (Hrs) % DNp73 remaining kDa kDa 58 58 30 30 46 46 Supplementary Figure 2 Bunjobpol et al.

3 (% vector control knockdown )
A B FosB c-Jun az1 Actin gapdh Vector Control si Az1 DNp73β (% vector control knockdown ) DNp73β/Actin Vector c-Jun FosB Control Az1 siRNA kDa kDa Az1 (p) Actin μM MDL 72527 58 17 30 46 46 30 46 Supplementary Figure 3 Bunjobpol et al.

4 Normailzed paox expression units
Thyroid Thyroid B-cell leukemia Prostate Gastric 3 2 2 2.5 2 1 1 1.5 Normailzed paox expression units 1.5 1 -2.5 Normal (n=4) Tumour (n=14) Normal (n=9) Tumour (n=9) Normal (n=74) Tumour (n=147) Normal (n=8) Tumour (n=13) Normal (n=31) Tumour (n=4) Oncomine boxed plots B kDa DNp73β Actin SHSY5Y DNp73β μM MDL 72527 58 46 Supplementary Figure 4 Bunjobpol et al.

5 Supplementary Figure 5 Bunjobpol et al.
SHSY5Y pcDNA SHSY5Y DNp73β +MDL Vector DNp73β SHSY5Y μM Doxorubicin + MDL 72527 - MDL 72527 Propidium Iodide Annexin V - FITC B C SHSY5Y pcDNA SHSY5Y DNp73β +MDL Caspase3 activity ΔOD400nm pcDNA DNp73β kDa 58 DNp73 Actin HCT116 46 D CDDP Control sipaox DNp73β HCT116 Vector Propidium Iodide Annexin V - FITC HCT116 pcDNA HCT116 DNp73β Control sipaox % Ann. V+/PI+ Supplementary Figure 5 Bunjobpol et al.

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