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Secretory kinase Fam20C tunes endoplasmic reticulum redox state via phosphorylation of Ero1α Protein immunoblotting of cell extracts and ConA precipitates.

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Presentation on theme: "Secretory kinase Fam20C tunes endoplasmic reticulum redox state via phosphorylation of Ero1α Protein immunoblotting of cell extracts and ConA precipitates."— Presentation transcript:

1 Secretory kinase Fam20C tunes endoplasmic reticulum redox state via phosphorylation of Ero1α
Protein immunoblotting of cell extracts and ConA precipitates from the conditioned medium of HeLa cells transfected with Ero1α (1) or Fam20C (2) alone, or co‐transfected with Ero1α and Fam20C (4), or co‐cultured cells expressing Ero1α and Fam20C separately (3). Immunofluorescence analysis showing the subcellular localization of p‐Ero1α in HepG2 cells co‐expressing Ero1α and Fam20C. PDI and GM130 were used as ER and Golgi markers, respectively. Scale bars = 10 μm. Protein immunoblotting of cell extracts and ConA precipitates from the conditioned medium of HeLa cells overexpressing Ero1α‐HA or Ero1α‐HA‐KDEL. Co‐immunoprecipitation of ERp44 and Ero1α/p‐Ero1α (Left) and protein immunoblotting of ConA precipitates from the conditioned medium (Right) in HeLa cells expressing Ero1α‐myc alone or with HA‐ERp44. Protein immunoblotting of cell extracts and ConA precipitates from the conditioned medium of HeLa cells transfected with Ero1α‐HA and scramble siRNA or siRNA targeting ERP44. Source data are available online for this figure. IF THIS IMAGE HAS BEEN PROVIDED BY OR IS OWNED BY A THIRD PARTY, AS INDICATED IN THE CAPTION LINE, THEN FURTHER PERMISSION MAY BE NEEDED BEFORE ANY FURTHER USE. PLEASE CONTACT WILEY'S PERMISSIONS DEPARTMENT ON OR USE THE RIGHTSLINK SERVICE BY CLICKING ON THE 'REQUEST PERMISSIONS' LINK ACCOMPANYING THIS ARTICLE. WILEY OR AUTHOR OWNED IMAGES MAY BE USED FOR NON-COMMERCIAL PURPOSES, SUBJECT TO PROPER CITATION OF THE ARTICLE, AUTHOR, AND PUBLISHER. EMBO J, Volume: 37, Issue: 14, First published: 01 June 2018, DOI: ( /embj )

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