1. A. Hi-II A
B. Hi-II B
C. Hi-II (A x B)
D. WCIC1
E. WCIC2
B73
Heterozygous
A188

2. Coleoptile removed and callus transferred to IM for 14 days25
50 zygotic embryos per plant placed on 2 tissue culture plates containing initiation/maintenance (IM) medium for 10 days
Coleoptile removed and callus transferred to IM for 14 days 10
Early measurements and selection:
Number of zygotic embryos displaying somatic embryogenesis was counted and average callus diameter of callus from three embryos measured per plate. Ten best embryos chosen by visual selection and kept on IM for 14 days 24 38
Late measurements and selection:
Number of zygotic embryos displaying somatic embryogenesis was counted and average callus diameter of three callus measured per plate. Callus was transferred to IM for 14 days 52
Callus was transferred to RM1 for 10 days
Days in culture 62
Callus was transferred to RM2 for 14 days
Number of embryos per plate per plant
Description of protocol steps