1. Fig. 1 Local COX-2 overexpression selectively increased the abundance and prolonged the presence of CD90+ mSSCs in the fractured bones.
Local COX-2 overexpression selectively increased the abundance and prolonged the presence of CD90+ mSSCs in the fractured bones. (A) B6 mice were subjected to femur fracture surgery (Fx). On the following day, animals received in fracture sites one of the following treatments (Tx): no treatment (No), a control AAV vector (Ctr), or the AAV.COX-2 vector (COX-2). On days 3, 4, 7 and 10 following the treatments, the fractured bones and contralateral intact bones were harvested and the MNCs were isolated as described in Materials and Methods. The isolated MNCs were analyzed by FACS. (B) Gating strategy. SSC, side scatter; FSC, forward scatter. (C) Representative FACS plots show the expressions of AlphaV (top), AlphaV and CD90 (middle), and AlphaV and 6C3 (bottom) among CD45−Tie2− cells. (D) Cumulative data show the frequencies of CD90+ mSSCs on day 7 following the treatments. (E) Representative FACS plots show the expressions of CD90 and 6C3 among mSSCs. (F) Representative FACS plots show the expressions of AlphaV and CD90 among CD45−Tie2− cells on days 3, 4, and 10 following the treatments. Where applicable, data are means ± SEM. **P < 0.01, ***P < 0.001, ****P < , #not significant; n = 3 to 5.
Samiksha Wasnik et al. Sci Adv 2019;5:eaaw2108
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