1. Fig. 6. Effect of CM from hASCs and melatonin-treated hASCs on cisplatin-induced apoptosis of HK-2 cells. HK-2 cells were exposed to either Media, NT-hASCs-CM, or Mel-hASCs-CM in the presence of cisplatin (With Cisplatin) at 7 μg/ml. HK-2 cells exposed to media and no cisplatin served as the control (Ctrl). After 20 h, a TdT-mediated dUTP nick-end labeling (TUNEL) assay was performed, cells were photographed, and the number of TUNEL-positive and 4′,6-diamidino-2-phenylindole (DAPI)-stained cells was determined as described in materials and methods. Photos (A) are from 1 representative example, and the graph (B) represents the results of experiments with 3 different hASCs preparations: Ctrl: 95% CI of the mean: 0.9–2.0 (n = 6); Media: 95% CI of the mean: 20.3–22.6 (n = 20); NT-hASCs-CM: 95% CI of the mean: 10.5–12.1 (n = 54); Mel-hASC-CM: 95% CI of the mean: 4.2–5.6 (n = 47). One-way ANOVA followed by Bonferroni's post hoc multiple comparisons test were conducted. Each P value was adjusted to account for multiple comparisons.
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