1. Depiction of wild-type and mutated Hes6 proteins.
Depiction of wild-type and mutated Hes6 proteins. A, Schematic representation of Hes6: shown are the N-terminal bHLH domain, followed by the helix3/helix4 domain containing the LNHLL motif, the putative PEST region, and the WRPW motif at the C-terminal end (Bae et al., 2000; Gratton et al., 2003). B, Hes6BAD contains a 35KKRR-to-35EKER mutation within the basic arm of the bHLH domain. C, Hes6AQ contains a two amino acid insertion (AQ) at position 39 at the end of the basic arm. D, Hes6LMD contains a 126LNHLL-to-126ANHAL mutation near the end of the helix3/helix4 domain. E, Hes6ΔWRPW lacks the last four C-terminal residues, WRPW. F, In the Hes6-VP16 protein, the WRPW motif was deleted and replaced with the VP16 transcription activation domain. G, The indicated FLAG-tagged Hes6 proteins were transfected into HEK293 cells, followed by Western blotting analysis (WB) with anti-FLAG antibody (Ab.).
Sumit Jhas et al. J. Neurosci. 2006;26:
©2006 by Society for Neuroscience