1. Fig. 2 NAD+-sensitized reduction of O2and oxidation of H2O.
NAD+-sensitized reduction of O2and oxidation of H2O. (A) Absorbance changes of NBT solution at 560 nm with varying concentration of NAD+. ΔA(t) ≡ A(t) − A(t = 0 min). The background signal [displayed in (B)] was not subtracted from ΔA(t). Reaction condition: NAD+ and NBT in an O2-purged sodium phosphate buffer (50 mM, pH 7.5) under irradiation (xenon lamp: λ, 260 to 900 nm and P, 200 mW cm−2). (B) A series of control experiments for each reaction component (i.e., 400 μM NAD+, light, and O2) in the photochemical formation of O2•−. ΔA ≡ A(30 min) − A(0 min). Reaction conditions: NAD+ and NBT in an O2-purged sodium phosphate buffer (50 mM, pH 7.5) under irradiation (xenon lamp: λ, 260 to 900 nm and P, 200 mW cm−2). (C) Effect of NAD+ concentration on OH• formation for 30-min irradiation (200 mW cm−2). (D) Dependency of OH• formation on the light intensity (t = 30 min). Reaction conditions: 1 mM NAD+ and Tris in an O2-purged sodium phosphate buffer (50 mM, pH 7.5). All reported values represent means ± SD (n = 3).
Jinhyun Kim et al. Sci Adv 2019;5:eaax0501
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