1. Figure 1. Effect of a peroxynitrite scavenger on high glucose-induced apoptosis in LLC-PK1 cells. Subconfluent monolayers of LLC-PK1 cells in 6-well tissue culture plates were exposed to 5 or 25 mM d-glucose for 48 h in the presence or absence of the peroxynitrite scavenger ebselen (10 μM, present throughout). A) Whole cell lysates used for evaluation of DNA fragmentation (ELISA). Results are expressed as fold of control ±se (48 h, n=6, *P<0.05). B) The same lysates were assayed for caspase-3 activity; results (±se) are expressed as fold of control (48 h, n=3, *P<0.05).
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