1. Supplementary figure 1 A B Water (Control) Pre-alcohol Post-alcohol
4 wks
3 wks
Sacrifice 1
Splenic injection
(MC38, 1×105 cells)
Water B
Alcohol
Water (Control)
Sacrifice 2
1 day
Splenic injection
(MC38, 2×105 cells)
4 wks
Sacrifice 3
Supplementary figure 1. Experimental models. (A) C57BL/6 mice were divided randomly into four groups (n=7-8 per group): normal group, pre-alcohol group, post-alcohol group, and alcohol group. Tumor injection (1×105 MC38 cells) was performed after 4 weeks of water (or alcohol) consumption. After an additional 3 weeks, the mice were sacrificed and liver metastases were evaluated (Sacrifice 1). (B) Two groups of mice were fed either water or 20 % alcohol for 4 weeks. The mice were sacrificed immediately for microenvironment-related assays (Sacrifice 2) or sacrificed 24 hours after splenic injection with fluorescence-labeled MC38 cells for the examination of cancer cell arrest (Sacrifice 3).