1. *p<0.05; **p<0.01; ***p<0.001.
Figure 5. The ADCC activity of cryopreserved NK cells in combination with rituximab in Raji lymphoma model. (A) In vitro ADCC activity of expanded NK cells with or without cryopreservation was assessed against Raji cells. Cryopreserved NK cells were used immediately after thawing without further culture or cytokine stimulation. Calcein-AM labeled Raji cells were co-cultured with NK cells for 4 h at E:T ratios of 30:1 to 0.3:1 in the presence of rituximab or huIgG. The percentage of specific lysis of Raji cells is expressed as the mean±standard error (n=6). (B, C) To determine in vivo ADCC activity of cryopreserved NK cells, Raji cells were intravenously transplanted into SCID mice and cryopreserved NK cells were administered 5 times at 2 to 3-day intervals. Cryopreserved NK cells were used immediately after thawing without further culture or cytokine stimulation. Rituximab was administered with NK cells together on day 1. Paralysis (%) (B) and survival rate (%) (C) were assessed. huIgG, human IgG.
*p<0.05; **p<0.01; ***p<0.001.
Figure 5. The ADCC activity of cryopreserved NK cells in combination with rituximab in Raji lymphoma model. (A) In vitro ADCC activity of expanded NK cells with or without cryopreservation was assessed against Raji cells. Cryopreserved NK cells were used immediately after thawing without further culture or cytokine stimulation. Calcein-AM…
Immune Netw Aug;18(4):e31.