1. A, Mean neurite length (± SEM) per motoneuron in 48 hr cultures after treatment with different concentrations of thrombin (1–1000 nm).
A, Mean neurite length (± SEM) per motoneuron in 48 hr cultures after treatment with different concentrations of thrombin (1–1000 nm). Thrombin was added 2 hr after the initial plating, and the cultures were stained with anti-β-tubulin antibodies 48 hr after the initial plating, as described in Materials and Methods. Control cultures were kept in L-15 media. B, The number of primary branches (mean ± SEM) that occurred on the longest neurite in the chick motoneuron cultures at 48 hr after treatment with thrombin. ***p ≤ for thrombin treatment versus control in A and B; n = 100 motoneurons examined per group.
Victoria L. Turgeon et al. J. Neurosci. 1998;18:
©1998 by Society for Neuroscience