1. Fig. 7. CIVO performance in canine lymphoma tumors demonstrates spatially restricted responses linked to drug mechanism.
CIVO performance in canine lymphoma tumors demonstrates spatially restricted responses linked to drug mechanism. (A to C) CIVO microinjection procedure adapted for a clinic-like, veterinary setting (A and B) by incorporating the placement of a “guide needle” into the optimal injection site using ultrasound guidance, (C) which aligned the microinjection device to target all needles of the handheld device into the optimal location in the tumor, increasing injection success. (D) ITD signal seen beneath the skin with a blue flashlight and yellow filter glasses, from a canine tumor injected using an eight-needle CIVO array. (E) Cancerous lymph nodes in two dogs were microinjected with the same amount of vincristine (1.5 μg) or a vehicle control. Tumors were resected 24 hours after microinjection. The ITD (green) and tumor responses (CC3, red; pHH3, yellow) were visualized in 4-μm sections from multiple depths along the injection column. Individual injection sites are shown for each dog. The fraction of biomarker-positive cells for each dog was plotted as a function of radial distance from the injection site. Data are average responses across three sections at about 2-mm intervals along the injection column ± SEM.
Richard A. Klinghoffer et al., Sci Transl Med 2015;7:284ra58
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