1. Multispectral Imaging Autofluorescence Microscopy in Colonic and Gastric Cancer Metastatic Lymph Nodes 
Desiree Pantalone, Francesco Andreoli, Franco Fusi, Venere Basile, Giovanni Romano, GianMario Giustozzi, Luigi Rigacci, Renato Alterini, Monica Monici 
Clinical Gastroenterology and Hepatology 
Volume 5, Issue 2, Pages (February 2007)
DOI: /j.cgh
Copyright © 2007 AGA Institute Terms and Conditions

2. Figure 1
AF spectra of bioptic sections from normal (continuous curve) and metastatic (dotted curve) lymph nodes and their difference (lower curve). AF spectra were recorded over a spot of about 30 μm in diameter, with a 2- to 4-second integration time, then corrected for the optical system spectral response and smoothed by a Gaussian convolution algorithm. After correction, spectra were normalized to their respective peaks as 100% for comparison.
Clinical Gastroenterology and Hepatology 2007 5, DOI: ( /j.cgh )
Copyright © 2007 AGA Institute Terms and Conditions

3. Figure 2
AF spectrum of a bioptic section from a metastatic lymph node. The primary tumor was a gastric carcinoma. Besides the principal peak at about 460 nm, a second peak at 602 nm can be observed.
Clinical Gastroenterology and Hepatology 2007 5, DOI: ( /j.cgh )
Copyright © 2007 AGA Institute Terms and Conditions

4. Figure 3
AF images of bioptic sections from (A) normal and (B–F) metastatic lymph nodes from GI tumors. For each sample, three 40-nm wide (full width at half maximum) spectral bands, peaking at about 450, 550, and 650 nm (450 FS 40-25, 550 FS 40-25, and 650 FS 40-25, respectively, Andover Corp.) were used to acquire 3 AF images sequentially with the same integration time. The overall acquisition was no longer than 12 seconds. Monochrome images then were combined in a single multicolored image using the red-green-blue technique.52 The multicolored images were obtained by the Image Combine Channels algorithm of Corel PHOTO-PAINT v 6.0 software (Corel Corporation, Ottawa, Canada), following identification of the 3 gray-scale images (acquired at 650, 550, and 450 nm) with the red-green-blue components.
Clinical Gastroenterology and Hepatology 2007 5, DOI: ( /j.cgh )
Copyright © 2007 AGA Institute Terms and Conditions