1. Expression of prostaglandin E2 receptor subtypes on cells in sputum from patients with asthma and controls: Effect of allergen inhalational challenge 
Sun Ying, MD, PhD, Brian J. O'Connor, MD, Qiu Meng, MD, Natalie Woodman, BSc, Steven Greenaway, MSc, Helen Wong, BSc, Kirsty Mallett, BSc, Tak H. Lee, MD, DSc, Chris Corrigan, MD, PhD 
Journal of Allergy and Clinical Immunology 
Volume 114, Issue 6, Pages (December 2004)
DOI: /j.jaci
Copyright © 2004 American Academy of Allergy, Asthma and Immunology Terms and Conditions

2. Fig 1
Single and double staining of EP1, EP2, and EP4 immunoreactive CD68+ macrophages (a-c), MBP+ eosinophils (d-f), and elastase+ neutrophils (g-i) in sputum from atopic patients with asthma. Black arrows, single immunocytochemistry (red) of CD68+ macrophages (a), MBP+ eosinophils (d), and elastase+ neutrophils (g). Green arrows, single immunocytochemistry (brown) of EP1(b), EP2(e), and EP4(h) immunoreactive cells. Black arrowheads, double staining (brown/red) of EP1+/CD68+ macrophages (c), EP2+/MBP+ eosinophils (f), and EP4+/elastase+ neutrophils (i).
Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci )
Copyright © 2004 American Academy of Allergy, Asthma and Immunology Terms and Conditions

3. Fig 2
Absolute numbers of EP1 through EP4(A-D, respectively) receptor-positive neutrophils (Neu), eosinophils (Eos), macrophages (Mac), lymphocytes (Lym), epithelial cells (Epi), and squamous cells (Squ) in sputum from normal controls (baseline, n=9 [x]; and 24 hours after saline challenge, n=3 [/]] and atopic patients with asthma (n=13) before (□) and 24 hours after (▪) allergen inhalational challenge. Bars show means ± SEMs.
Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci )
Copyright © 2004 American Academy of Allergy, Asthma and Immunology Terms and Conditions

4. Fig 3
Percentages of sputum cells of each phenotype accounting for the total EP1, EP2, EP3, and EP4(A-D, respectively) immunoreactive cells in normal controls (baseline, n=9 [x]; and 24 hours after saline challenge, n=3 [/]) and atopic patients with asthma (n=13) before (□) and 24 hours after (▪) allergen inhalational challenge. Bars show the means ± SEMs.
Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci )
Copyright © 2004 American Academy of Allergy, Asthma and Immunology Terms and Conditions

5. Fig 4
Percentages of cells expressing EP1 through EP4(A-D, respectively) receptor immunoreactivity in sputum from normal controls (baseline, n=9 [x]; and 24 hours after saline challenge, n=3 [/]) and atopic patients with asthma (n=13) before (□) and 24 hours after (▪) allergen inhalational challenge. Bars show the means ± SEMs.
Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci )
Copyright © 2004 American Academy of Allergy, Asthma and Immunology Terms and Conditions