1. Mature OSNs cannot engraft, and mature neurons are NQO1(+) and OCAM(+).
Mature OSNs cannot engraft, and mature neurons are NQO1(+) and OCAM(+). A, FACS strategy to isolate OMP-GFP(+)/pan-TdT(+) cells versus OMP-GFP (minus)/pan-TdT(+) cells (“All Else” cells). B, Quantification of the number of cells engrafted from OMP-GFP sorted and “All Else” cells. C, Host epithelium of sorted OMP-GFP transplants shows no graft-derived cells. D, Host epithelium from a transplant of “All Else” cells contains graft-derived OSNs. E–G, NQO1(+) cells do not overlap with Neurog1-eGFP(+) immature neuronal precursors (E) or Tuj1(+) immature OSNs (F), but do costain for the mature neuronal maker OMP (G). H–J, OCAM(+) cells overlap minimally with Neurog1-eGFP(+) cells (H) and Tuj1(+) immature OSNs (I), but the vast majority costain for the mature neuronal maker OMP (J). Dotted white line indicates the basal lamina. Scale bar, 20 μm.
Julie H. Coleman et al. J. Neurosci. 2019;39:
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