1. Forensic Characterization of Semen: 1. VISUALIZATION
MANY BODY FLUIDS FLUORESCE WITH ALTERNATE LIGHTING SOURCES

2. Forensic Characterization of Semen: 2. PRESUMPTIVE TESTING
ACID PHOSPHATASE ENZYME /
FOUND IN LARGE CONCENTRATIONS
IN SEMEN

3. Forensic Characterization of Semen: 3. CONFIRMATION
A. MICROSCOPIC IDENTIFICATION OF SPERM
B. DETECTION OF P30, A MALE PROSTATE PROTEIN. USEFULL FOR VASECTOMIZED MALES

4. Intact spermatozoa on a vaginal slide

5. Forensic Characterization of Saliva
Detection of Amylase
(breaks down starch in gel)
Evidence commonly tested for the presence of saliva includes:
Cigarette butts
Envelope flaps
Swabs taken from the body of sexual assault victims
Bottles, cans, & straws
Gel contains starch that is broken down (circles ) in the presence of Amylase enzyme

6. Nucleated epithelial cells from a cigarette butt

7. Microscopic detection of Hair shaft and root

8. Large piece of tissue from fingernail scrapings

9. Safety First—safeguards while handling biological evidence
Wear gloves
Keep contaminated surface away from face—protect those mucous membranes
Properly dispose of gloves/wash hands