1. Volume 19, Issue 11, Pages 1381-1390 (November 2012)
Identification of JadG as the B Ring Opening Oxygenase Catalyzing the Oxidative C-C Bond Cleavage Reaction in Jadomycin Biosynthesis 
Keqiang Fan, Guohui Pan, Xiaojing Peng, Jianting Zheng, Wubin Gao, Juan Wang, Weishan Wang, Yue Li, Keqian Yang 
Chemistry & Biology 
Volume 19, Issue 11, Pages (November 2012)
DOI: /j.chembiol
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2. Chemistry & Biology 2012 19, 1381-1390DOI: (10. 1016/j. chembiol. 2012
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3. Figure 1
Updated Summary of the Post-PKS Tailoring Reactions for the Biosynthesis of Atypical Angucyclines: Jadomycins, Gilvocarcins, and Kinamycins
Chemistry & Biology  , DOI: ( /j.chembiol )
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4. Figure 2
HPLC Traces of Dehydrorabelomycin Conversion Products at 313 nm
(A–C) Standards for: jadomycin B (2) (A); jadomycin A (3) (B); dehydrorabelomycin (8) (C).
(D–H) In vivo bioconversion of 8 by different strains: CH62 (ΔjadG) (D); VS668 (ΔjadH) (E); VS655 (ΔjadF) (F); G2006 (G); C2006 (H).
(I–P) In vitro enzymatic conversions using different combinations of JadG, JadY, Fre, and cofactors. The reaction mixture contained MOPS buffer (50 mM, pH 7.5), isoleucine (30 mM), FAD or FMN (1.1 μM), NADH or NADPH (166.7 μM), 8 (12.2 μM) and freshly purified enzymes. (I) JadG+FMN+NADH. (J) JadG+JadY+FMN+NADH. (K) JadG+JadY+FMN+NADPH. (L) JadG+JadY+FAD+NADH. (M) JadG+JadY+FAD+NADPH. (N) JadY+FMN+NADH. (O) JadG+Fre +FMN+NADH. (P) Fre+FMN+NADH.
See also Figure S3 and Table S1.
Chemistry & Biology  , DOI: ( /j.chembiol )
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5. Figure 3
In Vitro Reaction of JadY with FMN and NADH Monitored by Absorbance at 340 and 450 nm
The reaction mixture contained 50 mM MOPS, pH 7.5, 25 μM FMN, and 165 μM NADH. The reaction was triggered by injection of NADH solution at 30°C.
Chemistry & Biology  , DOI: ( /j.chembiol )
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6. Figure 4 Coupled Reactions Catalyzed by JadG and JadY/Fre
Chemistry & Biology  , DOI: ( /j.chembiol )
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