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Trained immunity effects on cell morphology and numbers.
Trained immunity effects on cell morphology and numbers. (A) Morphology of cells after 24 h of training and 6 days of rest when the cells were trained with RPMI (negative control), β-glucan, BCG, or oxLDL. Pictures were taken before restimulation at day 6. Magnification, ×20. (B) Size of cells after 24 h of training and 6 days of rest before restimulation at day 6 (n = 3, *, P < 0.05; compared to the RPMI control; ***, P < compared to the RPMI control). (C) Relative cell counts before restimulation at day 6 (n = 6; *, P < 0.05, compared to the RPMI control; ns, not significant). (D) IL-6 and TNF-α production after restimulation for corrected cell counts (n = 6; *, P < 0.05 compared to the RPMI control; **, P < 0.01 compared to the RPMI control). Siroon Bekkering et al. Clin. Vaccine Immunol. 2016; doi: /CVI
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