1. Volume 124, Issue 7, Pages 1891-1900 (June 2003)
Human cord blood-derived cells can differentiate into hepatocytes in the mouse liver with no evidence of cellular fusion 
Philip N Newsome, Ingolfur Johannessen, Shelagh Boyle, Evangelos Dalakas, Karen A Mcaulay, Kay Samuel, Frances Rae, Lesley Forrester, Marc L Turner, Peter C Hayes, David J Harrison, Wendy A Bickmore, John N Plevris 
Gastroenterology 
Volume 124, Issue 7, Pages (June 2003)
DOI: /S (03)

2. Figure 1
Glucose phosphate isomerase assay for in vivo detection of human cells. GPI analysis of bone marrow-derived samples from NOD-SCID mice infused with human cord blood cells for demonstration of the human isoenzyme. (A) Demonstrates the different electrophoretic mobility of human and NOD-SCID mouse iso-enzymes. (B) Shows the results obtained in a representative NOD-SCID mouse bone marrow after (labeled “recipient 1”) inoculation with a human cord blood sample. The results were in line with data obtained by FACS analysis and in vitro cultures.
Gastroenterology  , DOI: ( /S (03) )

3. Figure 2
Detection of human hepatocytes with immunohistochemistry. Immunohistochemistry on liver sections with antibody HepPar1 (brown) that detects human, but not mouse, hepatocytes. Cells were counterstained with Hematoxylin (blue). (A) Human liver. (B) Mouse liver. (C-F) Positive cells integrated into the hepatic plate of livers of NOD-SCID mice. (C and D) Represent the more typical finding of isolated single positive cells (higher magnification of C). (E and F) Represents the occasional clump of cells seen in the parenchyma. (Bar = 20 μm.)
Gastroenterology  , DOI: ( /S (03) )

4. Figure 3
Detection of human cells in liver sections by FISH. Detection of digoxigenin-labeled human DNA by FITC (green) in liver sections counterstained with DAPI (blue). Greyscale images of DAPI staining are shown on the right. (A) Human liver. (B) Mouse liver. (C and D) Human hepatocytic cells integrated into the hepatic plate of livers of NOD-SCID mice. (E and F) Human hematopoietic cells entering the murine liver. (G–I) Human CD34+ cells detected with immunohistochemistry entering the murine liver. ( J and K) Represent greyscale H&E pictures of cells with positive FISH signal and characteristic hepatocytic morphology. These cells had morphology typical of hepatocytes with large polyclonal size, large round nuclei, and complex intracellular cytoplasm. (Bar = 20 μm.)
Gastroenterology  , DOI: ( /S (03) )

5. Figure 4
Assessment of donor-host cell fusion. Simultaneous FISH analysis of liver sections with total human (green) and mouse (red) DNAs. Cells were counterstained with DAPI (blue). Greyscale images of the DAPI staining and mouse and human hybridization signals are shown. (A) Human liver. (B) Mouse liver. (C and D) Human positive (green) cells integrated into the hepatic plate of livers of NOD-SCID mice. Note the absence of any detectable mouse DNA (red) in these nuclei and their DAPI staining typical of human derived cells. (Bar = 20 μm.)
Gastroenterology  , DOI: ( /S (03) )