1. CD11c+ DCs from NOD.hCD205 mice are able to process and present Ag to diabetogenic CD8+ T cells.
CD11c+ DCs from NOD.hCD205 mice are able to process and present Ag to diabetogenic CD8+ T cells. (A–C) To assess the function of DCs from NOD.hCD205 mice, NOD or NOD.hCD205 CD11c+ DCs were treated with 1 μM NRP-V7, 1 μM YQLENYCAL (AL), 1 μg/ml anti–mCD205/NRP-V7, or appropriate controls in a 96-well plate. Either 8.3 (A and C) or AI4 (B) CD8+ T cells were added, and T cell proliferation was assessed by BrdU incorporation, which was quantified by ELISA. Graphs display mean absorbance ± SEM at the indicated DC:T cell ratios; each point indicates a mean of three replicates. (A) NOD or NOD.hCD205 DCs and 10, CD8+ T cells per well. (B) NOD or NOD.hCD205 DCs and 10,000 AI4 CD8+ T cells per well. (C) NOD.hCD205 DCs and 20, CD8+ T cells per well.
Jennifer Schloss et al. ImmunoHorizons 2019;3:
Copyright © 2019 The Authors