1. Deletion of VdHapX renders Verticillium dahliae more susceptible to H2O2.
Deletion of VdHapX renders Verticillium dahliae more susceptible to H2O2. (A) Conidial suspensions (1 × 105 spores) of each of the wild-type XS11, ΔVdHapX, and ΔVdHapX/VdHapX complemented strains were spread on potato dextrose agar plates. Sterile filter paper disks (5-mm diameter) were placed in the center of the plates, and 10 μl of an H2O2 solution (2.5%, 5%, and 10%) was added to each paper disk. The plates were incubated at 25°C for 4 days, and the inhibition zones were observed. (B) Bar chart of the inhibition zone of the above-described plates. Error bars represent the standard deviations based on three independent replicates. Asterisks indicate significant differences at P < (C) Expression of genes involved in H2O2 detoxification in V. dahliae, such as cat1, sod, sod_Cu, and yap1. Strains were grown in minimal medium for 3 days and then transferred for 45 min to 1 mM H2O2. Averages of the gene expression values were normalized against the V. dahliae β-tubulin gene. Error bars represent standard deviations. Asterisks represent significant differences (P < 0.01).
Yonglin Wang et al. mSphere 2018; doi: /mSphere