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Differences in proliferation of the hematopoietic cell line TF-1 and cytokine production by peripheral blood leukocytes induced by 2 naturally occurring.

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Presentation on theme: "Differences in proliferation of the hematopoietic cell line TF-1 and cytokine production by peripheral blood leukocytes induced by 2 naturally occurring."— Presentation transcript:

1 Differences in proliferation of the hematopoietic cell line TF-1 and cytokine production by peripheral blood leukocytes induced by 2 naturally occurring forms of human IL-3  Andrea Schweiger, MD, Debra Stern, MS, I.Carla Lohman, MS, Mauro Baldini, MD, Fernando D. Martinez, MD, Marilyn Halonen, PhD  Journal of Allergy and Clinical Immunology  Volume 107, Issue 3, Pages (March 2001) DOI: /mai Copyright © 2001 Mosby, Inc. Terms and Conditions

2 Fig. 1 The rhIL-3/P8 form of IL-3 (squares) induces enhanced proliferation of TF-1 cells compared with rhIL-3/S8 (circles) . TF-1 cells were cultured for 72 hours in triplicate. 5-Bromo-2’-deoxyuridine was added 8 hours before harvest. The number of experiments used at each concentration of IL-3 were 4 for 10–7 mol/L, 6 for 10–8 mol/L, and 7 for 10–7 mol/L and 10–12 mol/L. Absorbance units for control samples were ± *Significant difference in the 2 forms. All values for each form are significantly greater than control values. Journal of Allergy and Clinical Immunology  , DOI: ( /mai ) Copyright © 2001 Mosby, Inc. Terms and Conditions

3 Fig. 2 Human leukocytes primed with rhIL-3/S8 produce greater amounts of LTs compared with rhIL-3/P8. Dextran-separated cells were primed for 24 hours and stimulated with anti-IgE for 40 minutes before harvest. Each point represents the mean fold increase in IL-3–treated samples over control values (control RPMI) ± SEM for leukocytes from 8 donors. The production of LTs was undetectable without stimulation with anti-IgE (data not shown). *Significantly different amounts of LTs for the 2 forms of IL-3. †Significant difference from control values. Journal of Allergy and Clinical Immunology  , DOI: ( /mai ) Copyright © 2001 Mosby, Inc. Terms and Conditions

4 Fig. 3 Human leukocytes primed with rhIL-3/S8 produce greater amounts of IL-4 compared with rhIL-3/P8. Dextran-separated cells were primed for 1 (A) , 4 (B) , or 24 (C) hours and stimulated with anti-IgE for 40 minutes before harvest. Each point represents the mean fold increase over control (control RPMI) ± SEM of IL-4 production for 3 different donors. *Significant difference between the 2 forms of IL-3. †Significant difference from control values. Journal of Allergy and Clinical Immunology  , DOI: ( /mai ) Copyright © 2001 Mosby, Inc. Terms and Conditions

5 Fig. 4 IL-3 directly induces TNF-α production from human leukocytes. Cells were stimulated with (A) or without (B) anti-IgE. Each point represents the mean fold increase over control (control RPMI) ± SEM of TNF-α production for 6 donors. Values for rhIL-3/P8 were not different from control values. The data show that the production of TNF-α was not dependent on stimulation with anti-IgE (B ). *Significant differences between the 2 forms of IL-3. †Significant difference from control values. Journal of Allergy and Clinical Immunology  , DOI: ( /mai ) Copyright © 2001 Mosby, Inc. Terms and Conditions

6 Fig. 5 Polymyxin B does not inhibit the IL-3 enhancement over control of TNF-α secretion from human leukocytes. Leukocytes from one donor were stimulated with rhIL-3/S8 or rhIL-3/P8 at 10–8 mol/L in the presence or absence of polymyxin B at 10 or 20 μg and incubated for 24 hours. Journal of Allergy and Clinical Immunology  , DOI: ( /mai ) Copyright © 2001 Mosby, Inc. Terms and Conditions

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