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Optimized Allele-Specific Real-Time PCR Assays for the Detection of Common Mutations in KRAS and BRAF  Alois H. Lang, Heinz Drexel, Simone Geller-Rhomberg,

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Presentation on theme: "Optimized Allele-Specific Real-Time PCR Assays for the Detection of Common Mutations in KRAS and BRAF  Alois H. Lang, Heinz Drexel, Simone Geller-Rhomberg,"— Presentation transcript:

1 Optimized Allele-Specific Real-Time PCR Assays for the Detection of Common Mutations in KRAS and BRAF  Alois H. Lang, Heinz Drexel, Simone Geller-Rhomberg, Nicole Stark, Thomas Winder, Kathrin Geiger, Axel Muendlein  The Journal of Molecular Diagnostics  Volume 13, Issue 1, Pages (January 2011) DOI: /j.jmoldx Copyright © 2011 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

2 Figure 1 Primer and probes used for KRAS and BRAF real-time PCR. The figure illustrates positions of primer and probes used for KRAS and BRAF real-time PCR. Reference and mutation-specific PCRs share the same probe and the opposite PCR primer. Solid arrows display mutation-unspecific primers; and dotted arrows, mutation-specific primers. Codons affected by the mutation are underlined. The Journal of Molecular Diagnostics  , 23-28DOI: ( /j.jmoldx ) Copyright © 2011 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions


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