1. Do now
Directions: Describe three differences between the STRUCTURES of Animal and Plant Cells.
1______________________________
2_______________________________
3______________________________

2. List the three parts of the cell theory.
1______________________________
2_______________________________
3______________________________

3. Parts of a Compound Microscope
Objective:
Microscope care
Identify parts of the microscope
Calculate magnifications

4. Always carry with 2 hands
Microscope Care
Always carry with 2 hands
Only use lens paper for cleaning
Do not force knobs
Keep objects clear of desk and cords
Teacher demonstrates how to hold the microscope, where the lens paper is located and how to use it. Students will be invited to turn the knobs and observe the stage as it moves up and down. Teacher will demonstrate how to store the microscope.

5. Compound Microscope A microscope is a very powerful magnifying glass
A microscope helps you see things like cells up close

6. Eyepiece
View the specimen through the eyepiece

7. Stage Clips & Objectives
Stage clips hold the slide in place
Low power objective is used to focus the microscope (short & fat)
High power objective is used to view details of a specimen

8. Coarse Adjustment, Fine Adjustment, & Base
Coarse adjustment focuses adjustment
Fine adjustment fine tunes & gives detailed focus(usually smaller than coarse adjustment knob)
Base is where the microscope rests

9. Stage Stage is part where the slide rests
Mirror (or light source) directs light upwards onto the slide.

10. Diaphragm
Diaphragm allows light in

11. Arm
Arm is the part where you carry the microscope

12. Nosepiece
Nosepiece is the rotating device that holds the objectives (lenses)

13. Ocular & Objective lenses
Ocular lens – The lens that is in the eye piece.
Has a magnification of 10x.
Objective lens – The 3 lenses located on the nosepiece.
Each lens has a different magnification.
The total magnification of an image is calculated by multiplying the magnification of the ocular by the magnification of the objective.

14. Total magnifications ( Ocular lens = 10x )
Objective
Magnification
Total Magnification
Low 4X
40X
Medium
10X
100X
High
400X

15. Can you name the parts of a compound microscope?

16. Answers base mirror (light source) diaphragm stage stage clips
low power objective lens  
high power objective lens  
nosepiece  
arm 
fine focus knob  
body tube  
coarse focus knob  
eyepiece

17. Using the Microscope Place the Slide on the Microscope Use Stage Clips
Click Nosepiece to the lowest (shortest) setting
Look into the Eyepiece
Use the Coarse Focus
Give students a slide from the “common things” set, each student will practice focusing and changing objectives.

18. Using High Power Follow steps to focus using low power
Click the nosepiece to the longest objective
Do NOT use the Coarse Focusing Knob
Use the Fine Focus Knob to bring the slide
Have students exchange slides so they can look at different things, walk them through using the high power objective to focus slides. Emphasize not using the coarse objective during this process, as it will crack the slides.
What can you find on your slide?

19. FIELD OF VIEW

20. DO NOW: Is the field of view larger or smaller under high power?
Explain.

21. Field of View: Measurement of the area that can be seen.
How much can you see when you are 1 inch away from something?
How much can you see when you are 2 inches away from something?
How much can you see when you are 3 inches away from something?

22. How do we know how wide the field of view is?
We have to measure it while looking through the microscope.
4mm
4mm

24. 2 mm

25. 4 mm

26. how many boxes wide is the field of view?
3.5mm

28. What is the diameter of each field of view?B
5.5 3 C D
1.5 2

29. 4mm 1mm How big is the Paramecium?
When you place the grid on the stage and look at it under 40x, this is what you see.
When you look at a paramecium under 40 x, this is what you see
How big is the Paramecium?
4mm
1mm

30. The protist is about
2.5 mm.
5mm

31. Number that can fit across
Size of Object
Size of object =
Field of View
Number that can fit across

32. 9mm

33. Field of view
Number that can fit
3mm
3mm
3mm
9mm 3
= 3mm
9mm

34. Field of view
Number that can fit
3mm 5
0.6 mm
3mm

35. Which has a larger FOV?
100x
400x

36. Do Now Write down your homework
Put away everything except your lab and a pencil
Answer the prelab questions

37. Pre Lab Questions:
What are the three different total magnifications on our microscopes? 40
100
400

38. What is “Field of View”?
Measurement of the area that can be seen under the microscope.

39. What do you predict will be the relationship between power of lens and size of field of view?

40. Scanner Objective Lens Width of Diameter of FOV=
40 X
Low Power Objective Lens
100 X
High Power Objective Lens
400 X
Slide A
Grid
Diameter of FOV
(# of Boxes)
_________boxes
Width of Diameter of FOV=
___________mm 4
1.5 .5 4
1.5 .5

41. Exit Ticket: (tail not included)
How wide is the organism?

42. Do Now
Describe the proper way to find a specimen on high power.