1. Propofol modulates phasic and tonic GABAergic currents in spinal ventral horn interneurones 
V.S. Eckle, U. Rudolph, B. Antkowiak, C. Grasshoff 
British Journal of Anaesthesia 
Volume 114, Issue 3, Pages (March 2015)
DOI: /bja/aeu269
Copyright © 2015 The Author(s) Terms and Conditions

2. Fig 1
(a) Original whole-cell patch-clamp recording from one cell in organotypic spinal culture showing spontaneous GABAergic IPSCs. After establishing the control condition (upper traces), 1 µM propofol was applied (middle traces). Bicuculline abolished IPSC events completely (lower traces). (b) Averaged IPSC events from traces in (a). (c) Cumulative probability plot of IPSC decay values resulting from shown representative experiment. (d) Cumulative distribution of IPSC amplitudes from the same recording. Blue, control; green, propofol.
British Journal of Anaesthesia  , DOI: ( /bja/aeu269)
Copyright © 2015 The Author(s) Terms and Conditions

3. Fig 2
(a) Summary of the change in decay time with respect to control conditions. (b) Pooled data of amplitude of GABAergic IPSCs. (c) AUC derived from the decay time and amplitude values. (d) Representative traces from the same recording in Figure 1 and the counted IPSC events for each condition (marked as pink bars above the respective trace). (e) Cumulative distribution of IPSC frequency from one representative experiment (same cell as in d). (f) IPSC frequency. Number of experiments shown as insets.
British Journal of Anaesthesia  , DOI: ( /bja/aeu269)
Copyright © 2015 The Author(s) Terms and Conditions

4. Fig 3
(a) The effect of propofol on tonic GABAergic current (representative trace). Propofol (PRO, 1 µM) was applied with the selective glycine receptor antagonist strychnine plus tetrodotoxin (TTX). After 12 min, bicuculline was washed in. (b) All points count histogram of the same experiment in (a). (c) The effect of 5 µM propofol (representative trace). (d) All points count histogram of the same experiment as in (c). (e) Pooled data showing effects of propofol on induction of tonic GABA(A) current.
British Journal of Anaesthesia  , DOI: ( /bja/aeu269)
Copyright © 2015 The Author(s) Terms and Conditions

5. Fig 4
(a) Original traces from one representative extracellular recording showing spontaneous action potential firing under control conditions (upper trace) and after application of 1 µM propofol (lower trace) in the spinal ventral horn from wild-type mice. (b) Representative experiment from β3 (N265M) knock-in mice (β3 mutant), showing spontaneous action potential firing activity in control condition (upper trace) and propofol (lower trace). (c) Extracellular recordings of spontaneous action potential firing in the spinal ventral horn [blue line, filled circles, wild type; green line, open circles, β3 (N265M) knock-in mutant]. The clinically relevant range is displayed as a pink bar. Each data point (mean and sd) is from seven to 10 experiments.
British Journal of Anaesthesia  , DOI: ( /bja/aeu269)
Copyright © 2015 The Author(s) Terms and Conditions