1. The mutant p53‐ID4 complex controls VEGFA isoforms by recruiting lncRNA MALAT1
AWestern blot analysis of MDA‐MB‐468 cells interfered or not for hnRNP A1 expression using two different concentrations of siRNAs. BRT–qPCR analysis of two isoforms of aldolase A mRNA (ALDOA) differing for the inclusion/exclusion of exon 2. Ratio of exon 2 excluding isoform versus exon 2 including isoform is presented. Results from three biological replicates are shown. Data are presented as mean ± SEM. **P ≤ (two‐tailed Student's t‐test). C–HRepresentative Western blot experiments of SKBR3 (C, E, G), MDA‐MB‐231 (D), and MDA‐MB‐468 (F, G) cells transfected with the indicated siRNAs to p53, ID4, SRSF1, or MALAT1. Numbers indicate ratio between VEGFA165b protein densitometry values in interfered cells (si‐p53, si‐ID4, si‐SRSF1, si‐MALAT1) over si‐SCR sample normalized to total VEGFA protein levels. MALAT1 and ID4 depletion was assessed by RT–qPCR (H). Different siRNAs were used for each factor. Source data are available online for this figure.
IF THIS IMAGE HAS BEEN PROVIDED BY OR IS OWNED BY A THIRD PARTY, AS INDICATED IN THE CAPTION LINE, THEN FURTHER PERMISSION MAY BE NEEDED BEFORE ANY FURTHER USE. PLEASE CONTACT WILEY'S PERMISSIONS DEPARTMENT ON OR USE THE RIGHTSLINK SERVICE BY CLICKING ON THE 'REQUEST PERMISSIONS' LINK ACCOMPANYING THIS ARTICLE. WILEY OR AUTHOR OWNED IMAGES MAY BE USED FOR NON-COMMERCIAL PURPOSES, SUBJECT TO PROPER CITATION OF THE ARTICLE, AUTHOR, AND PUBLISHER.
EMBO Rep, Volume: 18, Issue: 8, Pages: , First published: 26 June 2017, DOI: ( /embr )