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Endogenous interleukin-4 and interleukin-10 regulate experimental lung ischemia reperfusion injury
Alexander S Farivar, MD, Baiya Krishnadasan, MD, Babu V Naidu, FRCS, Steven M Woolley, MRCS, Edward D Verrier, MD, Michael S Mulligan, MD, FACS The Annals of Thoracic Surgery Volume 76, Issue 1, Pages (July 2003) DOI: /S (03)
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Fig 1 Western blot analysis for interleukin-10. In the far left lane the molecular weight (MW) markers are represented, as referenced from the kaleidoscope marker. There is minimal interleukin-10 protein in negative controls and 1-hour reperfused lungs. At 2, 3, and 4 hours of reperfusion there is significantly increased expression of interleukin-10 protein in left lung homogenates. (hr = hour; kDa = kilodaltons.) The Annals of Thoracic Surgery , DOI: ( /S (03) )
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Fig 2 Western blot analysis for interleukin-4. In the far right lane the molecular weight (MW) markers are represented, as referenced from the kaleidoscope marker. There is no detectable interleukin-4 protein in negative controls or 1-hour reperfused lungs. At 2, 3, and 4 hours of reperfusion there is significant expression of interleukin-4 protein in left lung homogenates. (hr = hour; kDa = kilodaltons.) The Annals of Thoracic Surgery , DOI: ( /S (03) )
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Fig 3 Ribonuclease protection assay densitometry for cytokine mRNA using Image J software. Densitometric analysis reveals increased mRNA expression for tumor necrosis factor-α (■), interleukin-1β (□), and interferon-γ (▨) in animals treated with antibody to interleukin-4 and interleukin-10. (IL = interleukin.) The Annals of Thoracic Surgery , DOI: ( /S (03) )
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