1. Unanchored tri‐NEDD8 inhibits PARP‐1 to protect from oxidative stress‐induced cell death
WT and NEDP1 KO HEK 293 cells were transfected with FLAG‐NSUN2 and 48 h later harvested for anti‐FLAG immunoprecipitation. Following stringent washes, pulldowns were treated with recombinant GST‐NEDP1, the deubiquitinase his6‐USP1/UAF1, or left untreated. After incubation at 30°C for 30 min, reactions were stopped by the addition of LDS sample buffer, and then, samples were processed for Western blot analysis with the indicated antibodies. There is no shift in the anti‐FLAG immunoreactive band, which suggests NSUN2 is neither modified by NEDD8 or ubiquitin.
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EMBO J, Volume: 38, Issue: 6, First published: 25 February 2019, DOI: ( /embj )