1. Quantitative measurement of allele‐specific protein expression in a diploid yeast hybrid by LC‐MS
Accuracy of protein allele‐specific expression (pASE) measurements. (A) Direct comparison of the log2 ratios of chromatographic peak areas computed from two variant peptide pairs from the interspecies hybrid. (B) The log2 ratios of chromatographic peak areas measured between two distinct shared peptides (cyan) and S. cerevisiae (Scer) variant peptides (green) for the hybrid versus Scer sample. Each point corresponds to 404 proteins from technical replicate 1 for which pASE measurements were derived and two or more distinct shared and variant peptides were quantified. (C) The same plot for the hybrid versus S. bayanus (Sbay) sample for Sbay variant peptides (red) and shared peptides (cyan). The peptides compared are illustrated to the right. H, heavy and L, light reflect the isotope label that allows the two samples to be differentiated within an LC‐MS data set.
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Mol Syst Biol, Volume: 8, Issue: 1, First published: 14 August 2012, DOI: ( /msb )