1. a b c d e f Wankhade et al., Supplementary Figure 1
aP2 (Fabp4) protein expression
in mature adipocytes
TbRIAdKO
Gapdh
TbRI
adipocytes
SVC
TbRIAdWT
EWAT
AWAT
8 W
16 W NC
HFD
EWAT
Weeks on Normal Diet
Body Weight (Gm)
Total body weight
Body weight gain
Body weight and gain
Minutes after glucose injection
Glucose (mg/dl)
Glucose tolerance test
Daily food intake
Daily energy intake
Supplementary Figure 1
(a) aP2 (Fabp4) protein expression in mature adipocytes from EWAT and AWAT of 6 week old wild-type male mice fed normal chow (NC) or high-fat diet (HFD) for 8 weeks and 16 weeks. (b) TbRI levels in adipocytes and the SVC fraction from EWAT depots of TbR1AdKO mice and TbR1AdWT mice. The residual TbRI expression in the SVC fraction is due to presence of non-aP2-cre expressing cells that harbor intact TbRI. GAPDH levels are shown as protein loading control. (c, d) Body weight (c) and glucose tolerance (d) in TbR1AdKO (closed circle) mice and TbR1AdWT (open circle) mice fed normal chow diet over a 24 week period. (e, f) HFD food consumption in TbR1AdKO mice (closed circles) and TbR1AdWT (open circles) over (e) 2 week period (week 14 – 15) and (f) over 20 weeks of HFD feeding.

2. a b Wankhade et al., Supplementary Figure 2 EWAT UCP-1 (Abcam)
UCP-1 (Sigma)
TbRIAdWT
TbRIAdKO
UCP-1 (Abcam)
UCP-1 (Sigma)
BAT
BAT
Supplementary Figure 2
(a, b) UCP1 antibody comparison by immunohistochemistry using Sigma and Abcam antibodies on (a) EWAT from TβRIAdWT and TβRIAdKO mice and (b) BAT from TβRIAdWT . Same ratio of primary (1:500) and secondary antibody (1:1000) were used for both antibodies.

3. a b * c Wankhade et al., Supplementary Figure 3 TbRIAdWT TbRIAdKO BAT
H&E Staining
Adipocyte size in different depots
TbRIAdWT
TbRIAdKO
BAT
AWAT
MWAT
IWAT
RWAT
TbRIAdKO
TbRIAdWT * c
WT EWAT iBSCs stimulated with CL 316,243
Prdm16
Dio2
Ucp1
Cox8b 
Supplementary Figure 3
H&E staining (a) and adipocyte cell size (b) quantified in sections representing brown adipose tissue (BAT), anterior subcutaneous WAT (AWAT), mesenteric WAT (MWAT), inguinal WAT (IWAT), and retroperitoneal WAT (RWAT) in TbRIAdKO and TbRIAdWT mice on 24 weeks of HFD feeding. (c) Relative gene expression of beige/BAT specific markers in TbRIAdKO iBSCs after stimulation with CL Error Bars are expressed as +/- Standard Errors, (*p < 0.05; **p < 0.005; ***p < 0.001)

4. Thermal probe situated in graft
Wankhade et al., Supplementary Figure 4 d
Thermal probe situated in graft
Relative expression in recovered graft tissue compared with EWAT and BAT a
TbRIAdWT
TbRIAdKO b
SVCs c
TbRIAdWT
TbRIAdKO
iBSCs
Supplementary Figure 4
(a) Location of the thermal probe at the site of the developed graft. The IPTT-300 transponder (BioMedic Data Systems, Seaford, DE) was sutured at the site of transplant and the local temperature was detected with a scanner (DAS-7007; BioMedic Data Systems). (b-c) H & E staining (top panels) and UCP1 staining (bottom panels) in graft sections obtained from TbRIAdKO and TbRIAdWT SVCs (b) and iBSCs (c) after cold exposure (see details in methods section). (d) Individual gene expression levels from two transplanted mice for classic beige/BAT specific markers in EWAT, BAT and transplanted grafts from iBSCs (referred to as ADSCs here) harvested from TbRIAdKO and TbRIAdWT mice. Error bars are expressed as +/- Standard Errors, (*p < 0.05; **p < 0.005; ***p < 0.001).