1. Fig. 1. Stomatin expression in 3T3-L1 adipocytes
Fig. 1. Stomatin expression in 3T3-L1 adipocytes. A: cells were exposed to 25 mM glucose (G), 1 μM insulin in the presence of glucose (I), 25 mM fructose in the absence of glucose (F), or hexose-free medium (-H) for 12 h. Total RNA was collected, and 15 μg were separated on a 1% agarose gel. mRNA was probed for stomatin, GLUT1, and glucose-regulatory protein of 78 kDa (GRP78), as described in materials and methods. Ethidium bromide staining is shown as an indication of loading. B: cells were exposed to 25 mM glucose (+) or 25 mM fructose in the absence of glucose (-) for 12 h. Plasma membranes (PM), high-density membranes (HDM), and low-density membranes (LDM) were separated from fractionated cells. Proteins (50 μg) were separated by SDS-PAGE (12.5%) and analyzed for stomatin, Na+-K+-ATPase, GRP78, GLUT4, and GLUT1 expression by immunoblotting by use of a polyclonal antibody generated against specific sequences. Molecular mass markers in kDa are shown on the left.
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