1. Synergistic combination of valproic acid and oncolytic parvovirus H‐1PV as a potential therapy against cervical and pancreatic carcinomas
VPA treatment increases production of progeny virions Plaque assay. 1 × 106 HeLa cells were infected with H‐1PV (MOI of 0.01 or 0.1 pfu/cell) in the presence (black) or absence (white) of VPA (1 mM) for one production cycle. At 96 h post‐infection, the plaque assay was used to determine the number of viral particles in total cell lysates and culture medium. Average values from a typical experiment performed in triplicate are shown with standard deviation bars. Quantitative real time‐PCR. HeLa cells were treated as in panel A. At the indicated time points, cells were harvested and viral particles recovered from total cell lysates (left panel) or culture medium (right panel). Viral DNA was quantified by real time PCR. Numbers on top of the curves indicate the fold increase in virus titers in the presence versus absence of VPA. Southern blotting. Viral DNA was extracted from crude lysates of cells treated as described in panel A and analysed by Southern blotting. DNA samples were fractionated by agarose gel electrophoresis, transferred to a Hybond N nylon membrane and hybridized with an NS1‐specific DNA probe. Viral replicative‐form DNA was quantified by means of a PhosphorImager.
IF THIS IMAGE HAS BEEN PROVIDED BY OR IS OWNED BY A THIRD PARTY, AS INDICATED IN THE CAPTION LINE, THEN FURTHER PERMISSION MAY BE NEEDED BEFORE ANY FURTHER USE. PLEASE CONTACT WILEY'S PERMISSIONS DEPARTMENT ON OR USE THE RIGHTSLINK SERVICE BY CLICKING ON THE 'REQUEST PERMISSIONS' LINK ACCOMPANYING THIS ARTICLE. WILEY OR AUTHOR OWNED IMAGES MAY BE USED FOR NON-COMMERCIAL PURPOSES, SUBJECT TO PROPER CITATION OF THE ARTICLE, AUTHOR, AND PUBLISHER.
EMBO Mol Med, Volume: 5, Issue: 10, Pages: , First published: 17 September 2013, DOI: ( /emmm )