1. Structural and functional analysis of the GABARAP interaction motif (GIM)
Schematic presentation of the peptide array. Biotinylated LIR peptides were immobilized on the streptavidin‐coated 96‐well plate surface and treated with 6xHis‐tagged ATG8 proteins. To visualize binding, HRP‐coupled α‐HIS antibody solution was passed over the complexes and level of binding was measured as a light absorption at 450 nm (solely mediated by HRP‐coupled α‐HIS antibodies). Relative preferences of LC3B and LC3C proteins to the 30 LIR motifs tested. Absorbance of LC3C divided by absorbance of LC3B (cyan bars) and absorbance of LC3B divided by absorbance of LC3C (magenta bars) to define whether each LIR shows preference towards either LC3C or LC3B protein. Values are mean of n = 3 independent experiments ± SEM. Relative preferences of GABARAP and GABARAP‐L2 proteins to the 30 LIR motifs tested. Absorbance of GABARAP divided by absorbance of GABARAP‐L2 (cyan bars) and absorbance of GABARAP‐L2 divided by absorbance of GABARAP (green bars) to define whether each LIR shows preference towards either GABARAP or GABARAP‐L2 proteins. Values are mean of n = 3 independent experiments ± SEM.
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EMBO Rep, Volume: 18, Issue: 8, Pages: , First published: 27 June 2017, DOI: ( /embr )