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Structural and functional analysis of the GABARAP interaction motif (GIM) Interaction profile of 30 biotinylated LIR peptides from various proteins against.

Published byGeorgette Laurin Modified over 5 years ago

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Presentation on theme: "Structural and functional analysis of the GABARAP interaction motif (GIM) Interaction profile of 30 biotinylated LIR peptides from various proteins against."— Presentation transcript:

1 Structural and functional analysis of the GABARAP interaction motif (GIM)
Interaction profile of 30 biotinylated LIR peptides from various proteins against 6xHis‐tagged LC3B and 6xHis‐tagged GABARAP. Results for each LIR interactions were expressed as either absorbance of LC3B divided by absorbance of GABARAP (purple bars) or absorbance of GABARAP divided by absorbance of LC3B (blue bars) to define whether each LIR shows preference towards either LC3 or GABARAP family proteins. Dashed red line depicts 1.5‐fold change cut‐off. Values are mean of n = 3 independent experiments ± SEM. WebLogo generated from 14 sequences that showed preference towards GABARAP versus LC3B interaction. IF THIS IMAGE HAS BEEN PROVIDED BY OR IS OWNED BY A THIRD PARTY, AS INDICATED IN THE CAPTION LINE, THEN FURTHER PERMISSION MAY BE NEEDED BEFORE ANY FURTHER USE. PLEASE CONTACT WILEY'S PERMISSIONS DEPARTMENT ON OR USE THE RIGHTSLINK SERVICE BY CLICKING ON THE 'REQUEST PERMISSIONS' LINK ACCOMPANYING THIS ARTICLE. WILEY OR AUTHOR OWNED IMAGES MAY BE USED FOR NON-COMMERCIAL PURPOSES, SUBJECT TO PROPER CITATION OF THE ARTICLE, AUTHOR, AND PUBLISHER. EMBO Rep, Volume: 18, Issue: 8, Pages: , First published: 27 June 2017, DOI: ( /embr )

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