1. Endosome and Golgi‐associated degradation (EGAD) of membrane proteins regulates sphingolipid metabolism
See also Fig EV3. Epifluorescence and phase‐contrast microscopy of living WT yeast cells and the indicated mutants expressing GFP‐Orm2 or GFP‐Orm2‐K25,33R (green) with dsRed‐HDEL (red). Arrowheads point to GFP‐Orm2 accumulations outside the endoplasmic reticulum (ER). Epifluorescence and phase‐contrast microscopy of tul1Δ mutants expressing GFP‐Orm2 (green) with Vps4‐mCherry (red, endosomes) or with Sec7‐mCherry (red, Golgi) or Vps4‐eGFP (green) with Sec7‐mCherry. Left panel: epifluorescence and phase‐contrast microscopy of the indicated mutants expressing GFP‐Orm2 (green) with mCherry‐Cps1 (red, MVB cargo). E indicates class E compartments; right panel: Equal amounts of the indicated mutants in serial dilutions were incubated on agar plates at the indicated temperatures. Data information: scale bars = 5 μm.
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EMBO J, Volume: 38, Issue: 15, First published: 27 May 2019, DOI: ( /embj )