1. Functional assessment of pathogenic IgG subclasses in chronic autoimmune urticaria 
Sridharan Soundararajan, PhD, Yoko Kikuchi, DVM, Kusumam Joseph, PhD, Allen P. Kaplan, MD 
Journal of Allergy and Clinical Immunology 
Volume 115, Issue 4, Pages (April 2005)
DOI: /j.jaci
Copyright © 2005 American Academy of Allergy, Asthma and Immunology Terms and Conditions

2. Fig 1
Scheme for sequential isolation of IgG subclasses from purified patient IgG.
Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci )
Copyright © 2005 American Academy of Allergy, Asthma and Immunology Terms and Conditions

3. Fig 2
Concentration of individual IgG subclasses present within the IgG of a patient with chronic urticaria (A) and in the IgG2 preparation (B) isolated by antibody affinity chromatography.
Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci )
Copyright © 2005 American Academy of Allergy, Asthma and Immunology Terms and Conditions

4. Fig 3
Basophil histamine release after stimulation with whole IgG, isolated IgG2, and buffer control in 4 patients with autoimmune chronic urticaria and 1 with idiopathic chronic urticaria.
Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci )
Copyright © 2005 American Academy of Allergy, Asthma and Immunology Terms and Conditions

5. Fig 4
Isolation of IgG3 from 2 patients with autoimmune chronic urticaria. A and B, Concentration of IgG1 and IgG3 in the starting IgG, in the eluate containing IgG1, IgG2, and IgG4, and in the flow-through containing IgG3. C and D, Histamine release corresponding to each fraction.
Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci )
Copyright © 2005 American Academy of Allergy, Asthma and Immunology Terms and Conditions

6. Fig 5
Basophil histamine release after stimulation with isolated IgG subclasses from 3 patients with autoimmune chronic urticaria and 1 normal control.
Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci )
Copyright © 2005 American Academy of Allergy, Asthma and Immunology Terms and Conditions