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Bone marrow clonal T-cell population in lymphomatoid papulosis

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1 Bone marrow clonal T-cell population in lymphomatoid papulosis
Mitsuhito Ota, MD, Daisuke Sawamura, MD, PhD, Akihiko Shibaki, MD, PhD, Hiroshi Shimizu, MD, PhD  Journal of the American Academy of Dermatology  Volume 52, Issue 4, Pages (April 2005) DOI: /j.jaad Copyright © 2005 American Academy of Dermatology, Inc. Terms and Conditions

2 Fig 1 Analysis of the PCR products on a polyacrylamide gel. Each PCR reaction contained the following primers: Vβ1 (5′-TGT Ac/tC TCT GTG CCA GCA G-3′); Vβ2 (5′-TGT Ac/tC TCT GCA GTG CTA G-3′); Dβ1 (5′-CAA AGC TGT AAC ATT GTG GGG AC-3′); Dβ2 (5′-TCA TGG TGT AAC ATT GTG GGG AC-3′); Jβ1 (5′-ACA GTG AGC Ct/gG GTc/t CC-3′); and Jβ2 (5′-AGC ACt/c/g GTG AGC Ct/gG GTG CC-3′) to amplify TCR-β D-N-J fragments or V-N′-D-N-J fragments simultaneously. The arrow shows one clonally rearranged band in the skin lesion and the identical band within a smear background in the bone marrow sample. M, Size marker; Cs, skin negative control; CBM, bone marrow negative control. Journal of the American Academy of Dermatology  , DOI: ( /j.jaad ) Copyright © 2005 American Academy of Dermatology, Inc. Terms and Conditions


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