1. Depletion of CD4+ Cells Exacerbates the Cutaneous Response to Acute and Chronic UVB Exposure 
Jennifer L. Hatton, Allison Parent, Kathleen L. Tober, Tyler Hoppes, Brian C. Wulff, F. Jason Duncan, Donna F. Kusewitt, Anne M. VanBuskirk, Tatiana M. Oberyszyn 
Journal of Investigative Dermatology 
Volume 127, Issue 6, Pages (June 2007)
DOI: /sj.jid
Copyright © 2007 The Society for Investigative Dermatology, Inc Terms and Conditions

2. Figure 1
UVB exposure increases epidermal infiltration of CD4+ but not CD8+ T cells. Skh-1 hairless mice were selectively depleted of CD4+ or CD8+ cells before UVB as described in the Materials and Methods section. Quantitation of immunohistochemically stained sections (n=10 per group) was carried out to determine the number of epidermal infiltrating CD4+ (a) or CD8+ (b) cells. Error bars indicate SEM. (a) *P<0.02; **P<0.04; (b) *P<0.0006; **P<0.001.
Journal of Investigative Dermatology  , DOI: ( /sj.jid )
Copyright © 2007 The Society for Investigative Dermatology, Inc Terms and Conditions

3. Figure 2
Selective CD4+ cell depletion increases and prolongs UVB-induced MPO levels. The MPO assay was used to evaluate effects of CD4 and CD8 depletion on UVB-induced cutaneous inflammation (a) and to evaluate differences in the onset and length of the inflammatory response (b). Numbers of infiltrating LY6G positive neutrophils were counted in ten × 60 fields per section (c). (d) Representative photomicrographs of LY6-G staining taken at × 100 magnification (bar =20μm). Error bars indicate SEM. (a) *P<0.01, **P<0.004; (b) *P<0.001, **P<0.004, ***P<0.005; (c) *P<0.02, **P<0.04.
Journal of Investigative Dermatology  , DOI: ( /sj.jid )
Copyright © 2007 The Society for Investigative Dermatology, Inc Terms and Conditions

4. Figure 3
Effect of selective CD4+ cell depletion on PGE2 production and p53 induction in response to acute UVB exposure. PGE2 levels (a) in skin isolated from mice injected with control IgG antibody or anti-CD4 antibody before acute UVB exposure were quantitated using an enzyme-linked immunosorbent assay. *P<0.002; **P< (b) Graphical representation of %p53+ cells in the epidermis of treated mice. *P<0.04; **P< Error bars indicate SEM.
Journal of Investigative Dermatology  , DOI: ( /sj.jid )
Copyright © 2007 The Society for Investigative Dermatology, Inc Terms and Conditions

5. Figure 4
Selective depletion of neutrophils decreases UVB-induced epidermal p53. Skin of Skh-1 hairless mice selectively depleted of neutrophils was examined immunohistochemically and quantitated for (a) dermal neutrophil infiltration *P<0.0006, **P<0.0008; and (b) p53+ epidermal cells. *P<0.0002; **P< Error bars indicate SEM.
Journal of Investigative Dermatology  , DOI: ( /sj.jid )
Copyright © 2007 The Society for Investigative Dermatology, Inc Terms and Conditions

6. Figure 5
Effect of CD4+ cell depletion on UVB-induced tumor development. Mice were exposed to 2240J/m2 UVB three times weekly for 25 weeks. Beginning at week 11, mice were either injected with anti-CD4 antibodies (n=20) or control IgG antibodies (n=20) every 3 weeks. Half of the mice in each group were treated topically with 0.2ml acetone or 0.2ml celecoxib in acetone immediately following each UV exposure. *P<0.05 compared with UV/IgG/Ace; **P<0.05 compared with UV/IgG/Ace ***P<0.05 compared with UV/anti-CD4/Ace-treated groups. Error bars indicate SEM.
Journal of Investigative Dermatology  , DOI: ( /sj.jid )
Copyright © 2007 The Society for Investigative Dermatology, Inc Terms and Conditions