1. Absorbance (600 nm) Time (h) 1 0.1 0.01 5 10 15 20 25 305 10 15 20 25 30
Time (h)
Figure S1. Effects of metD (abc-yaeE-yaeC) operon expression in trans on
growth of the wild type, the abc-yaeE mutant and the yaeC mutant in M9
minimal medium as described in Figure 1. Ampicillin and arabinose were
added to the medium at 100 g/ml and 2 mM, respectively. , BW25113
(pBAD24); , BW25113 (pBAD24-metD); , abc-yaeE (pBAD24); , abc
-yaeE (pBAD24-metD); , yaeC (pBAD24); and , yaeC (pBAD24-metD).