1. Fig. 1 Caspase-8 and -9 are both activated during intrinsic and extrinsic apoptosis.
Caspase-8 and -9 are both activated during intrinsic and extrinsic apoptosis. (A and B). Western blot of NALM6 WT cells treated for the indicated time with (A) Birinapant (50 nM) and hTNF (10 ng/ml) or (B) with ABT263 (5 μM). (C) Western blot of control and CRISPR-knockout caspase-8– or -9–deficient leukemia cells; tubulin (Tub) is shown for loading control. (D and E) Activation of caspase-8 and -9, respectively, using caspase-8– or -9–specific FAM-FLICA stains and flow cytometry at indicated time points after treatment with (D) Birinapant (50 nM) and hTNF (10 ng/ml) (B + T) or (E) ABT263 (5 μM) (ABT) in WT or caspase-8 (C8 ko)– and caspase-9 (C9 ko)–deficient cells. (F) Model of apoptotic response activation of caspase-8 and -9 following extrinsic or intrinsic stimuli. Graphs show the mean ± SEM for three repeated experiments performed in duplicate.
Scott McComb et al. Sci Adv 2019;5:eaau9433
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