1. Fig. 3 DEP screening indicates positive correlation between S
Fig. 3 DEP screening indicates positive correlation between S. oneidensis polarizability and EET activity.
DEP screening indicates positive correlation between S. oneidensis polarizability and EET activity. (A) Schematic of the Mtr EET pathway in the S. oneidensis cell envelope. (B) Fe(III) citrate reduction over time measured for S. oneidensis WT strain MR-1, strain deficient in expressing both MtrABC and MtrDEF EET conduits (ΔMtr), and ΔMtr complemented with indicated proteins. Error bars indicate the SD. (C) Polarizability of the five S. oneidensis strains corresponding to (B) grown with different electron accepters, namely, (i) 60 mM pure fumarate and (ii) 15 mM Fe(III) citrate supplemented with a small amount (10 mM) of fumarate. Growth conditions in (B) and (C) (ii) are identical. The box-whisker plots indicate median and IQRs, with whiskers extending to 1.5 IQR below the 25th percentile and above the 75th percentile, respectively. Black crosses indicate outliers. Multiple comparison test of group means using one-way analysis of variance (ANOVA) suggests a significant difference (P < 0.05) between groups labeled with different letters. Asterisk indicates a significant difference (P < 0.03, two-tailed t test) between the polarizability of iron-reducing S. oneidensis (ii) and that of its fumarate-reducing counterpart (i).
Qianru Wang et al. Sci Adv 2019;5:eaat5664
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