1. Enzymes
Function and Kinetics
Recap

2. Theoretical Understanding of Enzyme Action
1-5
Theoretical Understanding of Enzyme Action
Does the rate of reaction depend on the free energy difference between S and P? No
What free energy differences define the equilibrium constant between S and P?
G product – Greactant = ΔGreaction
ΔGo = -RT ln Kequi
What free energy differences determine the rate of interconversion of between S and P?
ΔG of activation: ΔGact
Why do enzymes not alter the equilibrium constant, but change only the rate?
An enzyme can’t change ΔGreaction
It lowers the G of the transition state to increase the rate.

3. Basic Concepts of Enzyme Kinetics
6-13

4. Basic Concepts of Enzyme Kinetics
6-13

5. Basic Concepts of Enzyme Kinetics
6-13

6. Basic Concepts of Enzyme Kinetics
6-13

7. Basic Concepts of Enzyme Kinetics
6-13

8. Basic Concepts of Enzyme Kinetics
6-13

9. Basic Concepts of Enzyme Kinetics
6-13

10. Basic Concepts of Enzyme Kinetics
6-13

11. Basic Concepts of Enzyme Kinetics
6-13

12. Basic Concepts of Enzyme Kinetics
6-13

13. Basic Concepts of Enzyme Kinetics
6-13

14. Basic Concepts of Enzyme Kinetics
6-13

15. Basic Concepts of Enzyme Kinetics
6-13

20. 14-18
if [I] = Ki

21. 14-18

22. 14-18

23. 14-18

24. 14-18

25. 14-18

26. 14-18

27. 14-18

28. 14-18

29. 14-18

30. 19-23
if [I] = Ki

31. 19-23

32. 19-23

33. 19-23

34. 19-23

35. 19-23

36. 19-23

37. 19-23

38. 19-23

39. 19-23

40. Mixed Inhibitor 24

41. Mixed Inhibitor 24

42. a. No inhibitor
c. Competitive
b. Non-competitive
d. Mixed

43. Lineweaver-Burk
No inhibitor
Mixed
Non-competitive
Competitive