1. Tousled‐like kinase 2 regulates recovery from a DNA damage‐induced G2 arrest
AAsynchronously cycling U2OS cells were transfected with the indicated siRNAs and analyzed for outgrowth after 7 days using crystal violet staining. Hydroxyurea was added where indicated at a concentration of 1 mM. BQuantification of the experiment in (A). Error bars represent SD, n = 3. CAsynchronously cycling U2OS cells were treated as in (A). Cells were treated with EdU for 2 h, fixed, and analyzed for Edu incorporation. Scale bar indicates 10 μm. DQuantification of the experiment in (C). Error bars represent SD, n = 3. EWestern blot analysis of Tlk2 knockout cells. F, GIncorporation of the BlastR gene as measured by PCR amplification of genomic DNA. HCell cycle analysis of asynchronous parental and Tlk2Δ cell lines. IAsynchronously cycling parental and Tlk2Δ cell lines were treated with EdU for 4 h and analyzed for EdU incorporation similar to (D). Error bars represent SD, n = 3. JPopulation doubling during 3 consecutive days of asynchronously cycling parental and Tlk2Δ cell lines. Error bars represent SD, n = 3. KAdriamycin sensitivity in parental and Tlk2Δ cells. Cells were treated with the indicated amounts of adriamycin and analyzed for outgrowth using crystal violet staining after 7 days.
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EMBO Rep, Volume: 17, Issue: 5, Pages: , First published: 01 March 2016, DOI: ( /embr )